Herein we confirm the occurrence of Lophostoma carrikeri in Ecuador. One adult female was collected in Orellana province on 17 September 2012 at Boanamo, Waorani Ethnic Reserve, and another on 5 May 2013 at Yasuni Research Station, Yasuni National Park. These records extend the distributional range of the species about 570 km northwest of the nearest previously known record in Jenaro Herrera, Loreto, Peru. Both Ecuadorian localities are characterized by well-conserved primary Terra Firme rainforest. Morphological characters of the specimens presented here broaden the known intraspecific variation in this taxon, including uniformly dark ears without white margins, undeveloped sagittal crests, and smaller forearm size than previously reported. In addition, information on ectoparasites for this species in Ecuador is provided.