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      Survival, Persistence, and Isolation of the Emerging Multidrug-Resistant Pathogenic Yeast Candida auris on a Plastic Health Care Surface

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          ABSTRACT

          The emerging multidrug-resistant pathogenic yeast Candida auris represents a serious threat to global health. Unlike most other Candida species, this organism appears to be commonly transmitted within health care facilities and causes health care-associated outbreaks. To better understand the epidemiology of this emerging pathogen, we investigated the ability of C. auris to persist on plastic surfaces common in health care settings compared with that of Candida parapsilosis, a species known to colonize the skin and plastics. Specifically, we compiled comparative and quantitative data essential to understanding the vehicles of spread and the ability of both species to survive and persist on plastic surfaces under controlled conditions (25°C and 57% relative humidity), such as those found in health care settings. When a test suspension of 10 4 cells was applied and dried on plastic surfaces, C. auris remained viable for at least 14 days and C. parapsilosis for at least 28 days, as measured by CFU. However, survival measured by esterase activity was higher for C. auris than C. parapsilosis throughout the 28-day study. Given the notable length of time Candida species survive and persist outside their host, we developed methods to more effectively culture C. auris from patients and their environment. Using our enrichment protocol, public health laboratories and researchers can now readily isolate C. auris from complex microbial communities (such as patient skin, nasopharynx, and stool) as well as environmental biofilms, in order to better understand and prevent C. auris colonization and transmission.

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          Author and article information

          Contributors
          Role: Editor
          Journal
          J Clin Microbiol
          J. Clin. Microbiol
          jcm
          jcm
          JCM
          Journal of Clinical Microbiology
          American Society for Microbiology (1752 N St., N.W., Washington, DC )
          0095-1137
          1098-660X
          26 July 2017
          25 September 2017
          October 2017
          : 55
          : 10
          : 2996-3005
          Affiliations
          [a ]Mycotic Diseases Branch, Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, Atlanta, Georgia, USA
          [b ]Clinical and Environmental Microbiology Branch, Division of Healthcare Quality Promotion, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, Atlanta, Georgia, USA
          University of Iowa College of Medicine
          Author notes
          Address correspondence to Rory M. Welsh, mpo6@ 123456cdc.gov , or Anastasia P. Litvintseva, frq8@ 123456cdc.gov .

          Citation Welsh RM, Bentz ML, Shams A, Houston H, Lyons A, Rose LJ, Litvintseva AP. 2017. Survival, persistence, and isolation of the emerging multidrug-resistant pathogenic yeast Candida auris on a plastic health care surface. J Clin Microbiol 55:2996–3005. https://doi.org/10.1128/JCM.00921-17.

          Article
          PMC5625385 PMC5625385 5625385 00921-17
          10.1128/JCM.00921-17
          5625385
          28747370
          5467122d-9442-4c0d-9c6c-e407f818a39c
          This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.
          Page count
          supplementary-material: 3, Figures: 3, Tables: 3, Equations: 0, References: 32, Pages: 10, Words: 6662
          Categories
          Mycology
          Custom metadata
          October 2017

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