Genetic analysis of  Aegilops tauschii -derived seedling resistance to leaf rust in synthetic hexaploid wheat

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Abstract

Seedling resistance to leaf rust available in the synthetic hexaploid wheat line Syn137 was characterised by means of cytogenetic and linkage mapping. Monosomic analysis located a single dominant gene for leaf rust resistance on chromosome 5D. Molecular mapping not only confirmed this location but also positioned the gene to the distal part of the long arm of chromosome 5D. A test of allelism showed that the gene, tentatively named LrSyn137, is independent but closely linked to Lr1. It appears that Syn137 is occasionally heterogeneous for Lr1 since the analysis of the Lr1-specific marker RGA567-5 in the genetic mapping population indicated the presence of Lr1. Syn137 represents another source of genetic variation that can be useful for the diversification of leaf rust resistance in wheat cultivars.

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Leaf rust resistance gene Lr1, isolated from bread wheat (Triticum aestivum L.) is a member of the large psr567 gene family.

Robert Banks, Beat Keller, Brent D. McCallum … (2007)

In hexaploid wheat, leaf rust resistance gene Lr1 is located at the distal end of the long arm of chromosome 5D. To clone this gene, an F(1)-derived doubled haploid population and a recombinant inbred line population from a cross between the susceptible cultivar AC Karma and the resistant line 87E03-S2B1 were phenotyped for resistance to Puccinia triticina race 1-1 BBB that carries the avirulence gene Avr1. A high-resolution genetic map of the Lr1 locus was constructed using microsatellite, resistance gene analog (RGA), BAC end (BE), and low pass (LP) markers. A physical map of the locus was constructed by screening a hexaploid wheat BAC library from cultivar Glenlea that is known to have Lr1. The locus comprised three RGAs from a gene family related to RFLP marker Xpsr567. Markers specific to each paralog were developed. Lr1 segregated with RGA567-5 while recombinants were observed for the other two RGAs. Transformation of the susceptible cultivar Fielder with RGA567-5 demonstrated that it corresponds to the Lr1 resistance gene. In addition, the candidate gene was also confirmed by virus-induced gene silencing. Twenty T (1) lines from resistant transgenic line T (0)-938 segregated for resistance, partial resistance and susceptibility to Avr1 corresponding to a 1:2:1 ratio for a single hemizygous insertion. Transgene presence and expression correlated with the phenotype. The resistance phenotype expressed by Lr1 seemed therefore to be dependant on the zygosity status. T (3)-938 sister lines with and without the transgene were further tested with 16 virulent and avirulent rust isolates. Rust reactions were all as expected for Lr1 thereby providing additional evidence toward the Lr1 identity of RGA567-5. Sequence analysis of Lr1 indicated that it is not related to the previously isolated Lr10 and Lr21 genes and unlike these genes, it is part of a large gene family.

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Characterization and mapping of cryptic alien introgression from Aegilops geniculata with new leaf rust and stripe rust resistance genes Lr57 and Yr40 in wheat.

Vasu Kuraparthy, Muhammad Gill, Parveen Chhuneja … (2007)

Leaf rust and stripe rust are important foliar diseases of wheat worldwide. Leaf rust and stripe rust resistant introgression lines were developed by induced homoeologous chromosome pairing between wheat chromosome 5D and 5M(g) of Aegilops geniculata (U(g)M(g)). Characterization of rust resistant BC(2)F(5) and BC(3)F(6) homozygous progenies using genomic in situ hybridization with Aegilops comosa (M) DNA as probe identified three different types of introgressions; two cytologically visible and one invisible (termed cryptic alien introgression). All three types of introgression lines showed similar and complete resistance to the most prevalent pathotypes of leaf rust and stripe rust in Kansas (USA) and Punjab (India). Diagnostic polymorphisms between the alien segment and recipient parent were identified using physically mapped RFLP probes. Molecular mapping revealed that cryptic alien introgression conferring resistance to leaf rust and stripe rust comprised less than 5% of the 5DS arm and was designated T5DL.5DS-5M(g)S(0.95). Genetic mapping with an F(2)population of Wichita x T5DL.5DS-5M(g)S(0.95) demonstrated the monogenic and dominant inheritance of resistance to both diseases. Two diagnostic RFLP markers, previously mapped on chromosome arm 5DS, co-segregated with the rust resistance in the F(2) population. The unique map location of the resistant introgression on chromosome T5DL.5DS-5M(g)S(0.95) suggested that the leaf rust and stripe rust resistance genes were new and were designated Lr57 and Yr40. This is the first documentation of a successful transfer and characterization of cryptic alien introgression from Ae. geniculata conferring resistance to both leaf rust and stripe rust in wheat.

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Leaf Rust-Resistance Genes Lr41, Lr42, and Lr43 Transferred from Triticum tauschii to Common Wheat

W. J. Raupp, B Gill, T. Cox (1994)

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Contributors

Volker Mohler:

ORCID: http://orcid.org/0000-0001-5835-0925

volker.mohler@lfl.bayern.de

Journal

Journal ID (nlm-ta): J Appl Genet

Journal ID (iso-abbrev): J. Appl. Genet

Title: Journal of Applied Genetics

Publisher: Springer Berlin Heidelberg (Berlin/Heidelberg )

ISSN (Print): 1234-1983

ISSN (Electronic): 2190-3883

Publication date (Electronic): 24 January 2020

Publication date PMC-release: 24 January 2020

Publication date (Print): 2020

Volume: 61

Issue: 2

Pages: 163-168

Affiliations

[1 ]GRID grid.500031.7, ISNI 0000 0001 2109 6556, Bayerische Landesanstalt für Landwirtschaft (LfL), , Institut für Pflanzenbau und Pflanzenzüchtung (IPZ), ; Am Gereuth 8, 85354 Freising, Germany

[2 ]GRID grid.6936.a, ISNI 0000000123222966, Plant Breeding, Centre for Food and Life Sciences Weihenstephan, , Technical University of Munich, ; Liesel-Beckmann-Str. 2, 85354 Freising, Germany

Author notes

Communicated by: Barbara Naganowska

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Volker Mohler http://orcid.org/0000-0001-5835-0925

Article

Publisher ID: 541

DOI: 10.1007/s13353-020-00541-z

PMC ID: 7148280

PubMed ID: 31981185

SO-VID: 5468cd45-82e1-48a6-8f22-2144d6ebeae2

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

History

Date received : 5 November 2019

Date revision received : 19 December 2019

Date accepted : 8 January 2020

Funding

Funded by: Bayerische Landesanstalt für Landwirtschaft (4260)

Open Access :

Open Access funding provided by Projekt DEAL.

Custom metadata

ScienceOpen disciplines: Genetics

Keywords: disease resistance,molecular mapping,monosomic analysis,puccinia triticina,triticum aestivum

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ScienceOpen disciplines: Genetics

Keywords: disease resistance, molecular mapping, monosomic analysis, puccinia triticina, triticum aestivum

Genetic analysis of Aegilops tauschii-derived seedling resistance to leaf rust in synthetic hexaploid wheat

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Most cited references 20

Leaf rust resistance gene Lr1, isolated from bread wheat (Triticum aestivum L.) is a member of the large psr567 gene family.

Characterization and mapping of cryptic alien introgression from Aegilops geniculata with new leaf rust and stripe rust resistance genes Lr57 and Yr40 in wheat.

Leaf Rust-Resistance Genes Lr41, Lr42, and Lr43 Transferred from Triticum tauschii to Common Wheat

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