Draft Genome Sequences of Five Environmental Bacterial Isolates That Degrade Polyethylene Terephthalate Plastic

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Abstract

Here, we report the annotated draft genome sequences of three Pseudomonas spp. and two Bacillus spp. that, as consortia, degrade polyethylene terephthalate plastic. Improved microbial degradation of plastic waste could help reduce the billions of metric tons of these materials that currently exist in our environment.

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FQC Dashboard: integrates FastQC results into a web-based, interactive, and extensible FASTQ quality control tool

Joseph Brown, Meg Pirrung, Lee McCue (2017)

Abstract Summary FQC is software that facilitates quality control of FASTQ files by carrying out a QC protocol using FastQC, parsing results, and aggregating quality metrics into an interactive dashboard designed to richly summarize individual sequencing runs. The dashboard groups samples in dropdowns for navigation among the data sets, utilizes human-readable configuration files to manipulate the pages and tabs, and is extensible with CSV data. Availability and implementation FQC is implemented in Python 3 and Javascript, and is maintained under an MIT license. Documentation and source code is available at: https://github.com/pnnl/fqc. Contact joseph.brown@pnnl.gov

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Evidence of polyethylene biodegradation by bacterial strains from the guts of plastic-eating waxworms.

Jun Yang, Yu. Yang, Wei-Min Wu … (2014)

Polyethylene (PE) has been considered nonbiodegradable for decades. Although the biodegradation of PE by bacterial cultures has been occasionally described, valid evidence of PE biodegradation has remained limited in the literature. We found that waxworms, or Indian mealmoths (the larvae of Plodia interpunctella), were capable of chewing and eating PE films. Two bacterial strains capable of degrading PE were isolated from this worm's gut, Enterobacter asburiae YT1 and Bacillus sp. YP1. Over a 28-day incubation period of the two strains on PE films, viable biofilms formed, and the PE films' hydrophobicity decreased. Obvious damage, including pits and cavities (0.3-0.4 μm in depth), was observed on the surfaces of the PE films using scanning electron microscopy (SEM) and atomic force microscopy (AFM). The formation of carbonyl groups was verified using X-ray photoelectron spectroscopy (XPS) and microattenuated total reflectance/Fourier transform infrared (micro-ATR/FTIR) imaging microscope. Suspension cultures of YT1 and YP1 (10(8) cells/mL) were able to degrade approximately 6.1 ± 0.3% and 10.7 ± 0.2% of the PE films (100 mg), respectively, over a 60-day incubation period. The molecular weights of the residual PE films were lower, and the release of 12 water-soluble daughter products was also detected. The results demonstrated the presence of PE-degrading bacteria in the guts of waxworms and provided promising evidence for the biodegradation of PE in the environment.

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Author and article information

Contributors

Irene L. G. Newton: Role: Editor

Journal

Journal ID (nlm-ta): Microbiol Resour Announc

Journal ID (iso-abbrev): Microbiol Resour Announc

Journal ID (hwp): ga

Journal ID (pmc): mra

Journal ID (publisher-id): MRA

Title: Microbiology Resource Announcements

Publisher: American Society for Microbiology (1752 N St., N.W., Washington, DC )

ISSN (Electronic): 2576-098X

Publication date (Electronic): 20 June 2019

Publication date Collection: June 2019

Volume: 8

Issue: 25

Electronic Location Identifier: e00237-19

Affiliations

[a ]Department of Biology, Willamette University, Salem, Oregon, USA

[b ]Department of Biology, Reed College, Portland, Oregon, USA

Indiana University, Bloomington

Author notes

Address correspondence to Rosa León-Zayas, rleonzayas@ 123456willamette.edu .

Citation León-Zayas R, Roberts C, Vague M, Mellies JL. 2019. Draft genome sequences of five environmental bacterial isolates that degrade polyethylene terephthalate plastic. Microbiol Resour Announc 8:e00237-19. https://doi.org/10.1128/MRA.00237-19.

Article

Publisher ID: MRA00237-19

DOI: 10.1128/MRA.00237-19

PMC ID: 6588364

PubMed ID: 31221643

SO-VID: 54698e77-5776-4981-aeec-a83386aed52a

License:

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

History

Date received : 28 February 2019

Date accepted : 21 May 2019

Page count

Figures: 1, Tables: 1, Equations: 0, References: 7, Pages: 3, Words: 1683

Funding

Funded by: M.J. Murdock Charitable Trust (Murdock Trust), https://doi.org/10.13039/100000937;

Award ID: 2016189:MNL:11/17/2016

Award Recipient : Rosa Leon-Zayas

Custom metadata

cover-date June 2019

Data availability:

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