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      Superoxide–Mediated Glomerulopathy in the Endotoxin–Treated Pregnant Rat

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          Abstract

          In the present study the role of superoxide in the glomerular damage in the low–dose endotoxin–infused pregnant rats was investigated. On day 14 of pregnancy, 12 rats were infused for 1 h with 1.0 μg/kg bw endotoxin via a permanent jugular vein cannula. Of these rats, 6 were treated with SOD both prior to endotoxin infusion (7,000 U/kg) and 30 min (7,000 U/kg) and 4 h (14,000 U/kg) after the start of the infusion (SOD rats). The other 6 rats received no SOD treatment (endotoxin rats). Control pregnant rats were infused for 1 h with saline (saline rats; n = 6). Urinary albumin was measured on days 15 and 19 of pregnancy. On day 21, rats were sacrificed and kidney specimens were snap–frozen. Cryostat kidney sections were stained for fibrinogen, ecto–ATP diphosphohydrolase (e–ATPase) activity, polymorphonuclear cells, monocytes and various adhesion molecules on the endothelium and the leukocytes. SOD treatment appeared to significantly prevent the increased urinary albumin excretion and the decrease of glomerular e–ATPase activity which were observed in endotoxin–treated rats. This effect of SOD treatment after endotoxin infusion was associated with a significant inhibition of glomerular monocyte influx and a significant inhibition of adhesion molecule expression (glomerular ICAM–1 and VCAM–1 and leukocyte LFA–1 and VLA–4).The present data suggest that in the endotoxin–infused pregnant rat, production of superoxide in the first few hours after the infusion plays a role in the induction of glomerular damage, leading to albuminuria and diminished e–ATPase expression during the following days.

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          Most cited references 1

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          A method for frequent sampling of blood and continuous infusion of fluids in the rat without disturbing the animal

           A.B. Steffens (1969)
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            Author and article information

            Journal
            KBR
            Kidney Blood Press Res
            10.1159/issn.1420-4096
            Kidney and Blood Pressure Research
            S. Karger AG
            1420-4096
            1423-0143
            1998
            1998
            06 February 1999
            : 21
            : 6
            : 432-437
            Affiliations
            aDivision of Reproductive Biology, Department of Obstetrics and Gynaecology, and bDepartment of Pathology, University of Groningen, The Netherlands
            Article
            25896 Kidney Blood Press Res 1998;21:432–437
            10.1159/000025896
            9933828
            © 1998 S. Karger AG, Basel

            Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

            Page count
            Figures: 7, Tables: 1, References: 20, Pages: 6
            Product
            Self URI (application/pdf): https://www.karger.com/Article/Pdf/25896
            Categories
            Original Paper

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