+1 Recommend
1 collections
      • Record: found
      • Abstract: found
      • Article: found

      Quantitative RT-PCR for Neuroendocrine Studies

      Read this article at

          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.


          Determination of mRNA levels of specific genes is becoming increasingly important as a measure of gene expression. With the recent advent of RT-PCR, the sensitivity for mRNA determination has been increased dramatically, and this technique is becoming widely used in neuroendocrine studies which involve small tissue samples and/or isolated nuclei. Nevertheless, the exact procedure for reliable quantification of RT-PCR has been widely debated. This minireview attempts to assimilate the available literature on the RT-PCR technique and discuss the various approaches commonly used to obtain quantitative results using the technique. An example from our laboratory of the use of RT-PCR for the measurement of several gene products in the same sample using exogenous internal standards is also provided. Particular attention is paid to the choice of endogenous vs. exogenous internal standards, the length of the transcript of the standard and its relationship to the target sequence being amplified, the amplification pattern of the target gene and internal standard, the reproducibility of the method, and the overall usefulness and suitability of RT-PCR for neuroendocrine studies.

          Related collections

          Author and article information

          S. Karger AG
          09 April 2008
          : 63
          : 5
          : 397-407
          Department of Physiology and Endocrinology, Medical College of Georgia, Augusta, Ga., USA
          127065 Neuroendocrinology 1996;63:397–407
          © 1996 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 11


          Comment on this article