miR-491-3p suppresses the growth and invasion of osteosarcoma cells by targeting TSPAN1.

Abstract

Osteosarcoma (OS) is the most common primary bone cancer, and frequently leads to mortality as a result of rapid proliferation and metastasis. Recent data highlight the important role of microRNAs (miRs) in human cancers, including OS, providing a novel method for its diagnosis and treatment. In the present study, it was identified that miR‑491‑3p expression was frequently decreased in OS tissues and OS cell lines. Restored miR‑491‑3p expression suppressed the growth and invasion of OS cells. Bioinformatics and experimental analysis indicated that tetraspanin 1 (TSPAN1) is a direct target of miR‑491‑3p in OS. The TSPAN1 mRNA level was inversely associated with the miR‑491‑3p level in OS tissues and cell lines. Consistent with this, TSPAN1 knockdown inhibited the growth and invasion of OS cells. In conclusion, these data provide compelling evidence that miR‑491‑3p functions as a tumor suppressor in OS to attenuate the potential of growth and invasion by targeting TSPAN1.