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      A decade of 3C technologies: insights into nuclear organization.

      1 ,
      Genes & development
      Cold Spring Harbor Laboratory

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          Abstract

          Over the past 10 years, the development of chromosome conformation capture (3C) technology and the subsequent genomic variants thereof have enabled the analysis of nuclear organization at an unprecedented resolution and throughput. The technology relies on the original and, in hindsight, remarkably simple idea that digestion and religation of fixed chromatin in cells, followed by the quantification of ligation junctions, allows for the determination of DNA contact frequencies and insight into chromosome topology. Here we evaluate and compare the current 3C-based methods (including 4C [chromosome conformation capture-on-chip], 5C [chromosome conformation capture carbon copy], HiC, and ChIA-PET), summarize their contribution to our current understanding of genome structure, and discuss how shape influences genome function.

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          Author and article information

          Journal
          Genes Dev
          Genes & development
          Cold Spring Harbor Laboratory
          1549-5477
          0890-9369
          Jan 01 2012
          : 26
          : 1
          Affiliations
          [1 ] Hubrecht Institute-KNAW, University Medical Center Utrecht, CT Utrecht, The Netherlands.
          Article
          26/1/11
          10.1101/gad.179804.111
          3258961
          22215806
          5598467d-8f38-4d45-9e34-a51a92e73a11
          History

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