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      Regional differences in phylogenetic group of Escherichia coli strains isolated from children with urinary tract infection in Korea

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          Abstract

          Purpose

          We phylogenetically analyzed the Escherichia coli strains isolated from children with urinary tract infection (UTI) in 2 regions of Korea. Virulence factors (VFs) and antibiotic resistance of the strains were also determined to compare the possible differences.

          Methods

          A total of 138 E. coli strains were collected from the 2 regions; Gyeongin (78 strains) and Gyeongnam (60 strains). The phylogenetic groups were determined using the triplex polymerase chain reaction (PCR) method and multiplex PCRs were used to detect 7 VFs genes ( fimH, papC, iutA, hlyA, sfa/focDE, afa/draBC, and kpsMT II). We also tested for antibiotic resistance.

          Results

          Phylogenetic groups, B2 (61.6%) and D (26.8%), comprised the majority of all isolated strains. Regional comparisons revealed that more B2 strains and fewer non-B2 (A+B1+D) strains were found in Gyeongnam, than in the Gyeongin region ( P=0.033), and certain VFs were predominantly detected in Gyeongnam ( P<0.05). Neither regional nor phylogenetic differences, in antibiotic resistance of the strains, were significant.

          Conclusion

          We were able to confirm that the geographic location is an important determinant of the distribution of the phylogenetic groups and VFs among the E. coli strains that cause UTI in children.

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          Most cited references19

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          Proposal for a new inclusive designation for extraintestinal pathogenic isolates of Escherichia coli: ExPEC.

          T Russo, J Johnson (2000)
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            What defines extraintestinal pathogenic Escherichia coli?

            Christian-Daniel Köhler, Ulrich Dobrindt (2011)
            Escherichia coli (E. coli) exhibits considerable physiological and metabolic versatility and includes a variety of non-pathogenic, commensal variants, which belong to the normal gut flora of humans and warm-blooded animals. Additionally, several pathogenic variants have been identified which cause various types of intestinal or extraintestinal infections in humans and animals. In contrast to intestinal pathogenic E. coli (IPEC), which are obligate pathogens, extraintestinal pathogenic E. coli (ExPEC) are facultative pathogens which belong to the normal gut flora of a certain fraction of the healthy population where they live as commensals. Comparative genomics and epidemiological studies have been applied to study genomic diversity, markers, and phenotypic traits that may support discrimination of different E. coli pathotypes. Whereas IPEC are often epidemiologically and phylogenetically distinct from ExPEC and non-pathogenic, commensal strains, many ExPEC and non-pathogenic E. coli share large genomic fractions. Furthermore, extraintestinal infections of elderly or immunocompromised patients can be caused by E. coli variants which differ in their geno- and phenotypes from archetypal ExPEC. Thus, strain typing based on the detection of a limited number of ExPEC virulence/fitness-related genes may be ambiguous. A limited number of ExPEC-dominated clonal complexes can be identified in the E. coli population by multi locus sequence typing. Nevertheless, ExPEC and non-pathogenic E. coli cannot be clearly discriminated by molecular epidemiological approaches. Increased knowledge of the phylogeny, virulence and fitness traits, and host factors contributing to host susceptibility of the different groups of ExPEC variants is required for a better understanding of the biological basis of ExPEC infections. Copyright © 2011 Elsevier GmbH. All rights reserved.
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              Phylogenetic distribution of branched RNA-linked multicopy single-stranded DNA among natural isolates of Escherichia coli.

              P J Herzer, S Inouye, M Inouye … (1990)
              Multicopy single-stranded DNA (msDNA), a branched DNA-RNA molecule, has been shown in Escherichia coli B and clinical strain Cl-1 to be synthesized by reverse transcriptase. We report that 13% of the strains of the ECOR collection, a sample of 72 E. coli isolates representing the breadth of genetic variation of the species, produce msDNA. Three of the four major subspecific groups include msDNA-producing strains. Screening of 25 isolates that are genetically related to msDNA-producing clinical strains uncovered 22 additional msDNA-producing strains. A phylogenetic tree based on allelic variation detected electrophoretically at 20 enzyme-encoding loci revealed two major clusters and several deep branches composed of strains that synthesize msDNA. Although E. coli K-12 does not harbor msDNA, other closely related strains of the K-12 family do. The results support the hypothesis that msDNA-synthesizing systems, including reverse transcriptase genes, were acquired recently and independently in different lineages of E. coli.
              Article 0 comments Cited 82 times – based on 0 reviews     Review now
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                Author and article information

                Journal
                Journal ID (nlm-ta): Korean J Pediatr
                Journal ID (iso-abbrev): Korean J Pediatr
                Journal ID (publisher-id): KJP
                Title: Korean Journal of Pediatrics
                Publisher: The Korean Pediatric Society
                ISSN (Print): 1738-1061
                ISSN (Electronic): 2092-7258
                Publication date (Print): November 2012
                Publication date (Electronic): 23 November 2012
                Volume: 55
                Issue: 11
                Pages: 420-423
                Affiliations
                [1 ]Department of Pediatrics, The Catholic University of Korea College of Medicine, Seoul, Korea.
                [2 ]Department of Pediatrics, Seoul Adventist Hospital, Seoul, Korea.
                [3 ]Department of Pediatrics, Changwon Fatima Hospital, Changwon, Korea.
                Author notes
                Corresponding author: Soo Young Lee, MD, PhD. Department of Pediatrics, Incheon St. Mary's Hospital, The Catholic University of Korea College of Medicine, 56 Dongsu-ro, Bupyeong-gu, Incheon 403-720, Korea. Tel: +82-32-510-5687, Fax: +82-32-503-9724, sylee@ 123456catholic.ac.kr
                Article
                DOI: 10.3345/kjp.2012.55.11.420
                PMC ID: 3510271
                PubMed ID: 23227061
                SO-VID: 55be8c3a-a93f-433f-ac2d-661879a1cbb6
                Copyright © Copyright © 2012 by The Korean Pediatric Society
                License:

                This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                Date received : 25 April 2012
                Date revision received : 12 June 2012
                Date accepted : 01 July 2012
                Categories
                Subject: Original Article

                ScienceOpen disciplines: Pediatrics
                Keywords: child,escherichia coli,phylogeny,urinary tract infections
                Data availability:
                ScienceOpen disciplines: Pediatrics
                Keywords: child, escherichia coli, phylogeny, urinary tract infections

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