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      Cryobiotechnology of apple (Malus spp.): development, progress and future prospects

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          Stabilization of dry phospholipid bilayers and proteins by sugars.

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            Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification.

            The nucellar cells of navel orange(Citrus sinensis Osb. var. brasiliensis Tanaka) were successfully cryopreserved by vitrification. In this method, cells were sufficiently dehydrated with highly concentrated cryoprotective solution(PVS2) prior to direct plunge in liquid nitrogen. The PVS2 contains(w/v) 30% glycerol, 15% ethylene glycol and 15% DMSO in Murashige-Tucker medium(MT) containing 0.15 M sucrose. Cells were treated with 60% PVS2 at 25°C for 5 min and then chilled PVS2 at 0°C for 3 min. The cell suspension of about 0.1 ml was loaded in a 0.5 ml transparent plastic straw and directly plunged in liquid nitrogen for 30 min. After rapid warming, the cell suspension was expelled in 2 ml of MT medium containing 1.2 M sucrose. The average rate of survival was about 80%. The vitrified cells regenerated plantlets. This method is very simple and the time required for cryopreservation is only about 10 min.
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              Use of biotechnologies for the conservation of plant biodiversity

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                Author and article information

                Journal
                Plant Cell Reports
                Plant Cell Rep
                Springer Nature
                0721-7714
                1432-203X
                May 2018
                January 11 2018
                May 2018
                : 37
                : 5
                : 689-709
                Article
                10.1007/s00299-018-2249-x
                © 2018

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