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      Lipid and Protein Density in the Rat Retina: A Microradiographical Study

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          Abstract

          Lipid and protein density in each retinal layer were determined microradiographically in retinal sections of 10 rats. Lipid in retinal sections was extracted with chloroform. The fraction of dry mass remaining after lipid extraction is mainly protein. The dry mass lost in chloroform processing was calculated to obtain the lipid density, which accounts for about 30% of the total dry mass density. The total dry mass density in each retinal layer is the sum of the lipid and protein density. Lipid should be regarded as an important X-ray absorber when determining the dry mass density in the rat retina with microradiography.

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          Author and article information

          Journal
          ORE
          Ophthalmic Res
          10.1159/issn.0030-3747
          Ophthalmic Research
          S. Karger AG
          0030-3747
          1423-0259
          1991
          1991
          10 December 2009
          : 23
          : 4
          : 220-224
          Affiliations
          Department of Medical Biophysics, Karolinska Institute and Eye Laboratory, St. Eriks Eye Hospital, Stockholm, Sweden
          Article
          267106 Ophthalmic Res 1991;23:220–224
          10.1159/000267106
          1945293
          © 1991 S. Karger AG, Basel

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          Pages: 5
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