Mikania glomerata (Asteraceae) hosts eight gall midge species (Diptera: Cecidomyiidae), each one responsible for a different gall morphotype. In a survey conducted in Recanto das Cigarras, Viçosa, Minas Gerais state (Brazil), we found three of these eight gall midges: Liodiplosis cylindrica, L. spherica, and Asphondylia glomeratae, each exhibiting a low infestation rate. This study offers an updated geographic distribution of these three gall midge species, recorded for the first time in Minas Gerais state.