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      The Vacuum Assisted Negative Pressure Isolation Hood (VANISH) System: Novel Application of the Stryker Neptune™ Suction Machine to Create COVID-19 Negative Pressure Isolation Environments

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          Abstract

          Coronavirus disease 2019 (COVID-19) may remain viable in the air for up to three hours, placing health care workers in close proximity to aerosolizing procedures particularly at high risk for infection. This combined with the drastic shortage of negative pressure rooms hospitals worldwide has led to the rapid innovation of novel biohazard isolation hoods, which can be adapted to create negative pressure isolation environments around the patient's airway using the hospital wall suction, which carries many limitations, including weaker suction capabilities, single patient use, and immobility. Here, we report our Vacuum Assisted Negative Pressure Isolation Hood (VANISH) system that uses a mobile and readily available in most hospital operating rooms Stryker Neptune™ (Stryker Corporation, Kalamazoo, Michigan) high-powered suction system to more effectively create a negative pressure biohazard isolation environment. VANISH has been utilized regularly in an anesthesia practice of 30+ providers and, to date, there have been no documented COVID-19 infections.

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          Aerosol and Surface Stability of SARS-CoV-2 as Compared with SARS-CoV-1

          To the Editor: A novel human coronavirus that is now named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (formerly called HCoV-19) emerged in Wuhan, China, in late 2019 and is now causing a pandemic. 1 We analyzed the aerosol and surface stability of SARS-CoV-2 and compared it with SARS-CoV-1, the most closely related human coronavirus. 2 We evaluated the stability of SARS-CoV-2 and SARS-CoV-1 in aerosols and on various surfaces and estimated their decay rates using a Bayesian regression model (see the Methods section in the Supplementary Appendix, available with the full text of this letter at NEJM.org). SARS-CoV-2 nCoV-WA1-2020 (MN985325.1) and SARS-CoV-1 Tor2 (AY274119.3) were the strains used. Aerosols (<5 μm) containing SARS-CoV-2 (105.25 50% tissue-culture infectious dose [TCID50] per milliliter) or SARS-CoV-1 (106.75-7.00 TCID50 per milliliter) were generated with the use of a three-jet Collison nebulizer and fed into a Goldberg drum to create an aerosolized environment. The inoculum resulted in cycle-threshold values between 20 and 22, similar to those observed in samples obtained from the upper and lower respiratory tract in humans. Our data consisted of 10 experimental conditions involving two viruses (SARS-CoV-2 and SARS-CoV-1) in five environmental conditions (aerosols, plastic, stainless steel, copper, and cardboard). All experimental measurements are reported as means across three replicates. SARS-CoV-2 remained viable in aerosols throughout the duration of our experiment (3 hours), with a reduction in infectious titer from 103.5 to 102.7 TCID50 per liter of air. This reduction was similar to that observed with SARS-CoV-1, from 104.3 to 103.5 TCID50 per milliliter (Figure 1A). SARS-CoV-2 was more stable on plastic and stainless steel than on copper and cardboard, and viable virus was detected up to 72 hours after application to these surfaces (Figure 1A), although the virus titer was greatly reduced (from 103.7 to 100.6 TCID50 per milliliter of medium after 72 hours on plastic and from 103.7 to 100.6 TCID50 per milliliter after 48 hours on stainless steel). The stability kinetics of SARS-CoV-1 were similar (from 103.4 to 100.7 TCID50 per milliliter after 72 hours on plastic and from 103.6 to 100.6 TCID50 per milliliter after 48 hours on stainless steel). On copper, no viable SARS-CoV-2 was measured after 4 hours and no viable SARS-CoV-1 was measured after 8 hours. On cardboard, no viable SARS-CoV-2 was measured after 24 hours and no viable SARS-CoV-1 was measured after 8 hours (Figure 1A). Both viruses had an exponential decay in virus titer across all experimental conditions, as indicated by a linear decrease in the log10TCID50 per liter of air or milliliter of medium over time (Figure 1B). The half-lives of SARS-CoV-2 and SARS-CoV-1 were similar in aerosols, with median estimates of approximately 1.1 to 1.2 hours and 95% credible intervals of 0.64 to 2.64 for SARS-CoV-2 and 0.78 to 2.43 for SARS-CoV-1 (Figure 1C, and Table S1 in the Supplementary Appendix). The half-lives of the two viruses were also similar on copper. On cardboard, the half-life of SARS-CoV-2 was longer than that of SARS-CoV-1. The longest viability of both viruses was on stainless steel and plastic; the estimated median half-life of SARS-CoV-2 was approximately 5.6 hours on stainless steel and 6.8 hours on plastic (Figure 1C). Estimated differences in the half-lives of the two viruses were small except for those on cardboard (Figure 1C). Individual replicate data were noticeably “noisier” (i.e., there was more variation in the experiment, resulting in a larger standard error) for cardboard than for other surfaces (Fig. S1 through S5), so we advise caution in interpreting this result. We found that the stability of SARS-CoV-2 was similar to that of SARS-CoV-1 under the experimental circumstances tested. This indicates that differences in the epidemiologic characteristics of these viruses probably arise from other factors, including high viral loads in the upper respiratory tract and the potential for persons infected with SARS-CoV-2 to shed and transmit the virus while asymptomatic. 3,4 Our results indicate that aerosol and fomite transmission of SARS-CoV-2 is plausible, since the virus can remain viable and infectious in aerosols for hours and on surfaces up to days (depending on the inoculum shed). These findings echo those with SARS-CoV-1, in which these forms of transmission were associated with nosocomial spread and super-spreading events, 5 and they provide information for pandemic mitigation efforts.
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            Aerodynamic analysis of SARS-CoV-2 in two Wuhan hospitals

            The ongoing outbreak of coronavirus disease 2019 (COVID-19) has spread rapidly on a global scale. Although it is clear that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is transmitted through human respiratory droplets and direct contact, the potential for aerosol transmission is poorly understood1-3. Here we investigated the aerodynamic nature of SARS-CoV-2 by measuring viral RNA in aerosols in different areas of two Wuhan hospitals during the outbreak of COVID-19 in February and March 2020. The concentration of SARS-CoV-2 RNA in aerosols that was detected in isolation wards and ventilated patient rooms was very low, but it was higher in the toilet areas used by the patients. Levels of airborne SARS-CoV-2 RNA in the most public areas was undetectable, except in two areas that were prone to crowding; this increase was possibly due to individuals infected with SARS-CoV-2 in the crowd. We found that some medical staff areas initially had high concentrations of viral RNA with aerosol size distributions that showed peaks in the submicrometre and/or supermicrometre regions; however, these levels were reduced to undetectable levels after implementation of rigorous sanitization procedures. Although we have not established the infectivity of the virus detected in these hospital areas, we propose that SARS-CoV-2 may have the potential to be transmitted through aerosols. Our results indicate that room ventilation, open space, sanitization of protective apparel, and proper use and disinfection of toilet areas can effectively limit the concentration of SARS-CoV-2 RNA in aerosols. Future work should explore the infectivity of aerosolized virus.
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              Barrier Shields: Not Just for Intubations in Today’s COVID-19 World?

              To the Editor The coronavirus disease (COVID-19) pandemic has led to awareness of the heightened risk for the anesthesia provider. A recent joint position statement by the American Society of Anesthesiologists, the Anesthesia Patient Safety Foundation, the American Academy of Anesthesiologist Assistants, and the American Association of Nurse Anesthetists recommended as optimal practice that all anesthesia professionals utilize personal protective equipment (PPE) appropriate for aerosol-generating procedures for all patients. 1 However, there remains a shortage of PPE, and some institutions still limit the use of N95 respirators and powered air-purifying respirators (PAPRs) for confirmed COVID cases. Because of this scarcity, physical barriers have been proposed as a means of protecting personnel during airway instrumentation. The first “aerosol box” was designed and shared on social media by a Taiwanese anesthesiologist, Dr Hsien-Yung Lai. 2 This transparent plastic cube was designed to allow the patient to lie at the head of the operating room table, separated from the anesthesia provider by a clear barrier, with 2circular openings at the superior end to allow the clinician’s hands to pass through and perform airway manipulation. Canelli et al 3 recently demonstrated that a simulated cough resulted in contamination of the inner surface of the box and the laryngoscopist’s gloves and gowned forearms, as opposed to pollution of the operating room environment more than 2 m away when no barrier was utilized. With current lack of widespread point-of-care testing, there remains the risk of transmission from asymptomatic carriers. 4 Moreover, during surgery, other aerosol-generating procedures might be required intraoperatively and at emergence, including the nebulization of inhaled beta-agonist, emergent reintubation, oropharyngeal suctioning, extubation of the endotracheal tube, and delivery of high-flow oxygen. These same measures are also performed during monitored anesthesia care (MAC) cases. In addition, certain types of procedures typically done under MAC, such as upper endoscopy, can cause a high incidence of coughing. Depending on the stability of the structure, intubation shields may be left at the head of the operating room table for the duration of the surgical procedure as opposed to immediately removed after successful endotracheal intubation. A small clear drape can be used to cover the ports while allowing the anesthesia provider ready access to the patient’s airway (Figure). At the end of the case, these physical barriers can be easily cleaned with spray disinfectant and/or germicidal disposable wipes. As with all novel techniques, there remains a learning curve to familiarize oneself for use in everyday practice. Finally, in certain cases, the patient’s body habitus, anatomical location of the surgery, or surgical positioning may preclude the use of the intubation shield. Figure. A clear drape covers the 2 circular openings of the intubation shield during surgery. With a limited supply of PPE, the intubation shield or other barrier devices could be a reasonable cost-effective strategy to help protect anesthesia professionals and other surgical personnel, and their usage should be considered for all cases currently being performed in the operating room. ACKNOWLEDGMENTS The author thanks Collectible Grading Authority (Norcross, GA) for the donation of the intubation shields. Phil B. Tsai, MD, MPH Department of Anesthesiology Rancho Los Amigos National Rehabilitation Center Downey, California ptsai@dhs.lacounty.gov
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                Author and article information

                Journal
                Cureus
                Cureus
                2168-8184
                Cureus
                Cureus (Palo Alto (CA) )
                2168-8184
                14 May 2020
                May 2020
                : 12
                : 5
                : e8126
                Affiliations
                [1 ] Anesthesiology and Critical Care, Massachusetts General Hospital, Boston, USA
                [2 ] Anesthesiology, Mercy General Hospital, Sacramento, USA
                [3 ] Anesthesia, Critical Care, and Pain Medicine, Massachusetts General Hospital, Boston, USA
                Author notes
                Article
                10.7759/cureus.8126
                7228800
                570098ef-45e2-498a-8144-060f961f1ac1
                Copyright © 2020, Convissar et al.

                This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                : 1 May 2020
                : 13 May 2020
                Categories
                Anesthesiology
                Infectious Disease
                Quality Improvement

                coronavirus disease (covid-19),negative pressure rooms,negative pressure hood,negative pressure tent,health care worker safety,covid-19,wall suction,stryker neptune,vanish system,vacuum assisted negative pressure isolation hood

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