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      In vitro shoot regeneration from roots and leaf discs of Passiflora cincinnata mast.

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          Abstract

          Different concentrations of 6-benzyladenine (6-BA) were used in vitro to induce buds in three types of explants: leaf discs, root segments and the seedling itself obtained from in vitro seed germination of Passiflora cincinnata Mast. The 0.5 mg.L -1 6-BA concentration was most suitable for all the three explants, however, bud formation time and means (direct/indirect) were different for each type of explant. In seedlings used as explants, it had shoot regeneration from the primary root. Histological events leading to bud formation via meristemoids were described. The origin was indirect in leaf discs, with callus formation in subepidermal cells of the chlorophyll parenchyma layers. In root segments and in seedling roots, the buds were formed directly in the pericycle (in roots with some secondary structure) and in the vascular cambium (at the initial phase of secondary structure). Also, indirect buds originated from meristemoids which were formed around the callus.

          Translated abstract

          Diferentes concentrações de 6-benziladenida (6-BA) foram usadas para a indução de brotos in vitro em três tipos de explantes: discos foliares, segmentos de raiz e plântula obtidos da germinação de sementes in vitro de Passiflora cincinnata Mast. A concentração de 0,5 mg.L-1 foi a mais adequada para os três explantes, porém, o tempo e a via (direta/indireta) de formação da gema diferiu para cada tipo de explante. Nas plântulas utilizadas como explante houve a formação de gemas a partir da raiz primária. Os eventos histológicos que levaram a formação de um novo órgão, via meristemóides (centros meristemáticos) foram descritos. Nos discos foliares a origem foi indireta, com formação de calo a partir das células subepidérmicas das camadas de parênquima clorofiliano. Nos segmentos de raiz e nas raízes das plântulas, a organogênese direta apresentou duas origens, a partir do periciclo, nas raízes com início da estrutura secundária, e a partir do câmbio vascular, nas raízes com estrutura secundária já estabelecida. Também nos segmentos de raiz observou-se a via indireta cujo calo resultou da proliferação do periciclo.

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          Most cited references 24

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          Simple method for differential staining of paraffin embedded plant material using toluidine blue o.

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            A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy

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              Axillary bud development of passionfruit as affected by ethylene precursor and inhibitors

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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                babt
                Brazilian Archives of Biology and Technology
                Braz. arch. biol. technol.
                Instituto de Tecnologia do Paraná - Tecpar (Curitiba )
                1678-4324
                March 2007
                : 50
                : 2
                : 239-247
                Affiliations
                [1 ] Universidade de São Paulo Brazil
                [2 ] Instituto Agronômico de Campinas
                [3 ] Universidade de São Paulo Brazil
                Article
                S1516-89132007000200009
                10.1590/S1516-89132007000200009
                Product
                Product Information: website
                Categories
                BIOLOGY

                General life sciences

                organogenesis, Anatomical, 6-benzyladenine, passionfruit

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