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      Iodinated Bovine Prolactin

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          Iodinated bovine prolactin (2.6 iodine atoms/molecule; labelled with a trace of <sup>125</sup>I to give a specific activity of 0.041 µCi/mg) was prepared by the chloramine T method. It was active in two bioassays (pigeon crop sac and dispersed mouse mammary cell), though somewhat less active than the unmodified hormone. In an immunoassay, iodinated prolactin was more effective than the unmodified hormone at displacing <sup>125</sup>I-prolactin from antibody. High specific activity <sup>125</sup>I-prolactin (1 iodine atom/molecule; 70 µCi/µg) was used for autoradiographic studies on the binding of prolactin to mouse mammary cells. In vivo the labelled hormone found in the mammary gland was associated with membranes of mammary epithelial cells and with alveolar lumen contents. In vitro <sup>125</sup>I-prolactin was shown to bind to dispersed mouse mammary epithelial cells.

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          Author and article information

          Horm Res Paediatr
          Hormone Research in Paediatrics
          S. Karger AG
          25 November 2008
          : 8
          : 1
          : 37-50
          Biochemistry Laboratory, School of Biological Sciences, University of Sussex, Falmer, Brighton
          178778 Horm Res 1977;8:37–50
          © 1977 S. Karger AG, Basel

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          Pages: 14


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