Chromatin remodelers Isw1 and Chd1 maintain chromatin structure during transcription by preventing histone exchange

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Abstract

Set2-mediated methylation of histone H3 Lys36 (H3K36) is a mark associated with the coding sequences of actively transcribed genes, yet plays a negative role during transcription elongation. It prevents trans-histone exchange over coding regions and signals for histone deacetylation in the wake of RNA polymerase II (RNAPII) passage. We have found that in Saccharomyces cerevisiae the Isw1b chromatin-remodeling complex is specifically recruited to open reading frames (ORFs) by H3K36 methylation through the PWWP domain of its Ioc4 subunit in vivo and in vitro. Isw1b acts in conjunction with Chd1 to regulate chromatin structure by preventing trans-histone exchange from taking place over coding regions and thus maintains chromatin integrity during transcription elongation by RNA polymerase II.

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Most cited references 35

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The biology of chromatin remodeling complexes.

Cedric R Clapier, Bradley R Cairns (2009)

The packaging of chromosomal DNA by nucleosomes condenses and organizes the genome, but occludes many regulatory DNA elements. However, this constraint also allows nucleosomes and other chromatin components to actively participate in the regulation of transcription, chromosome segregation, DNA replication, and DNA repair. To enable dynamic access to packaged DNA and to tailor nucleosome composition in chromosomal regions, cells have evolved a set of specialized chromatin remodeling complexes (remodelers). Remodelers use the energy of ATP hydrolysis to move, destabilize, eject, or restructure nucleosomes. Here, we address many aspects of remodeler biology: their targeting, mechanism, regulation, shared and unique properties, and specialization for particular biological processes. We also address roles for remodelers in development, cancer, and human syndromes.

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Journal

Journal ID (nlm-journal-id): 101186374

Journal ID (pubmed-jr-id): 31761

Journal ID (nlm-ta): Nat Struct Mol Biol

Journal ID (iso-abbrev): Nat. Struct. Mol. Biol.

Title: Nature structural & molecular biology

ISSN (Print): 1545-9993

ISSN (Electronic): 1545-9985

Publication date Nihms-submitted: 4 August 2012

Publication date (Electronic): 26 August 2012

Publication date (Print): September 2012

Publication date PMC-release: 01 March 2013

Volume: 19

Issue: 9

Pages: 884-892

Affiliations

[1 ]Stowers Institute for Medical Research, 1000 E 50 ^th Street, Kansas City, Missouri 64110, USA

[2 ]Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas 66160, USA

Author notes

Correspondence should be addressed to J.L.W. ( jlw@ 123456stowers.org )

Article

Manuscript ID: NIHMS382271

DOI: 10.1038/nsmb.2312

PMC ID: 3560298

PubMed ID: 22922743

SO-VID: 57ae2c78-1295-43e7-ac8a-9b3425026da5

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Chromatin remodelers Isw1 and Chd1 maintain chromatin structure during transcription by preventing histone exchange

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Abstract

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Higher order chromatin architecture

Most cited references 35

The biology of chromatin remodeling complexes.

Dissecting the regulatory circuitry of a eukaryotic genome.

R: A Lenguage and Environment for Statisctical Computing

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