A single-stranded junction modulates nanosecond motional ordering of the substrate recognition duplex of a group I ribozyme.

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Abstract

Rigid spinning: Site-directed spin-labeling studies using a rigid nitroxide spin label (Ç) reveal that both length and sequence of a single-stranded junction (J1/2) modulate nanosecond motional ordering of the substrate-recognition duplex (P1) of the 120 kD group I ribozyme. The studies demonstrate an approach for experimental measurements of nanosecond dynamics in high-molecular-weight RNA complexes.

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Author and article information

Journal

Journal ID (iso-abbrev): Chembiochem

Title: Chembiochem : a European journal of chemical biology

ISSN (Electronic): 1439-7633

ISSN (Print): 1439-4227

Publication date (Electronic): Sep 23 2013

Volume: 14

Issue: 14

Affiliations

[1 ] Department of Chemistry, University of Southern California, LJS-251, 840 Downey Way, Los Angeles, CA 90089-0744 (USA) http://pzqin.usc.edu/pzqhome.

Article

Mid ID: NIHMS512386

DOI: 10.1002/cbic.201300376

PMC ID: 3817310

PubMed ID: 23900919

SO-VID: 57be0eaa-c3e0-41dc-a75b-457ecbc3c271

History

Keywords: EPR spectroscopy,RNA,dynamics,motional ordering,spin labeling

Data availability:

Keywords: EPR spectroscopy, RNA, dynamics, motional ordering, spin labeling

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