Transcriptome-wide mining suggests conglomerate of genes associated with tuberous root growth and development in Aconitum heterophyllum Wall

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Abstract

Tuberous roots of Aconitum heterophyllum constitute storage organ for secondary metabolites, however, molecular components contributing to their formation are not known. The transcriptomes of A. heterophyllum were analyzed to identify possible genes associated with tuberous root development by taking clues from genes implicated in other plant species. Out of 18 genes, eight genes encoding GDP-mannose pyrophosphorylase (GMPase), SHAGGY, Expansin, RING-box protein 1 (RBX1), SRF receptor kinase (SRF), β-amylase, ADP-glucose pyrophosphorylase (AGPase) and Auxin responsive factor 2 (ARF2) showed higher transcript abundance in roots (13–171 folds) compared to shoots. Comparative expression analysis of those genes between tuberous root developmental stages showed 11–97 folds increase in transcripts in fully developed roots compared to young rootlets, thereby implying their association in biosynthesis, accumulation and storage of primary metabolites towards root biomass. Cluster analysis revealed a positive correlation with the gene expression data for different stages of tuberous root formation in A. heterophyllum. The outcome of this study can be useful in genetic improvement of A. heterophyllum for root biomass yield.

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Most cited references 37

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Functional genomic analysis of the AUXIN RESPONSE FACTOR gene family members in Arabidopsis thaliana: unique and overlapping functions of ARF7 and ARF19.

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Author and article information

Contributors

Rajinder Singh Chauhan: +91 1792 239231 , rajinder.chauhan@juit.ac.in

Journal

Journal ID (nlm-ta): 3 Biotech

Journal ID (iso-abbrev): 3 Biotech

Title: 3 Biotech

Publisher: Springer Berlin Heidelberg (Berlin/Heidelberg )

ISSN (Electronic): 2190-5738

Publication date (Electronic): 11 July 2016

Publication date PMC-release: 11 July 2016

Publication date (Print): December 2016

Volume: 6

Issue: 2

Electronic Location Identifier: 152

Affiliations

Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, Himachal Pradesh 173234 India

Article

Publisher ID: 466

DOI: 10.1007/s13205-016-0466-y

PMC ID: 4940232

SO-VID: 57d36cbc-bb12-4f94-b754-91bb935ea34e

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Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

History

Date received : 17 April 2016

Date accepted : 28 June 2016

Funding

Funded by: FundRef http://dx.doi.org/10.13039/501100001407, Department of Biotechnology , Ministry of Science and Technology;

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Keywords: aconitum heterophyllum,biomass,primary metabolites,transcriptome,transcript abundance,tuberous root

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Keywords: aconitum heterophyllum, biomass, primary metabolites, transcriptome, transcript abundance, tuberous root

Transcriptome-wide mining suggests conglomerate of genes associated with tuberous root growth and development in Aconitum heterophyllum Wall

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Most cited references 37

Rapid isolation of high molecular weight plant DNA.

Functional genomic analysis of the AUXIN RESPONSE FACTOR gene family members in Arabidopsis thaliana: unique and overlapping functions of ARF7 and ARF19.

Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development.

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