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      DIMA Replication in Pancreatic Islets and Adrenals in Diabetic Chinese Hamsters

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          Abstract

          The diabetic syndrome in Chinese hamsters is progressive and there is a decrease in the size of the pancreatic islets. To what extent this is due to impaired DNA replication in their islet cells is unknown. Therefore <sup>3</sup>H-thymidine incorporation into islet cells was studied in genetically diabetic (subline L) male hamsters aged 17-20 days, 6 weeks, 3, 6 and 12 months. Because adrenal weight was decreased at almost all ages, studies of adrenal morphology and function were also performed. Age- and sex-matched normal (subline M) hamsters served as controls. Autoradiography showed progressively falling labeling with increasing age in both normal and diabetic animals in both islets and adrenals. At the earliest age studied, the diabetic subline animals were normoglycemic but showed hyperinsulinemia. DNA replication was reduced both in the islet cells and the adrenal cortex. From 6 weeks of age and onwards, the diabetic hamsters were hyperglycemic and usually hyperinsulinemic but DNA replication in both islets and adrenals was not different from that in normals. Histomorphometry showed reduced volume in both the adrenal medulla and cortex in 3-month-old diabetic hamsters. In 12-month-old male and female diabetic hamsters plasma cortisol and aldosterone and 24-hour urinary epinephrine were normal, but urinary norepinephrine and dopamine levels were excessive. Thus, in contrast to what is found in the obese-hyperglycemic mouse, there was no increased islet cell DNA replication in response to the increased functional demand in the diabetic Chinese hamster. The transiently decreased DNA replication in the youngest animals may contribute to the reduced islet volume later in life. Also, in contrast to the findings in obese-hyperglycemic mice, adrenal weights were decreased and adrenal-cortex function appeared to be normal in the diabetic hamsters. Perhaps this lack of adrenal enlargement is one significant factor for the lack of adaptive islet growth in the diabetic hamster.

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          Author and article information

          Journal
          HRE
          Horm Res Paediatr
          10.1159/issn.1663-2818
          Hormone Research in Paediatrics
          S. Karger AG
          1663-2818
          1663-2826
          1993
          1993
          03 December 2008
          : 39
          : 1-2
          : 67-72
          Affiliations
          aDepartment of Histology and Cell Biology, University of Umeå, and bDepartment of Medical Cell Biology, Uppsala University, Uppsala, Sweden
          Article
          182698 Horm Res 1993;39:67–72
          10.1159/000182698
          8406343
          © 1993 S. Karger AG, Basel

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          Page count
          Pages: 6
          Categories
          Original Paper

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