2
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Recurrent variation in the active NOR sites in the monkey frogs of the genus Pithecopus Cope, 1866 (Phyllomedusidae, Anura)

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Abstract

          Treefrogs of the genus Pithecopus Cope, 1866 exhibit expressive chromosomal homogeneity which contrasts with a high variation frequency of the nucleolus organizer region (NOR) related to the group. Currently, the genus contains eleven species and no chromosomal data are available on P. palliatus Peters, 1873, P. ayeaye Lutz, 1966 and P. megacephalus Miranda-Ribeiro, 1926. Here, we describe the karyotypes of these three species based on Giemsa staining, C-banding, silver impregnation and in situ hybridization (FISH). We were also analyze the evolutionary dynamic of the NOR-bearing chromosome in species of genus under a phylogenetic view. The results indicate that P. palliatus , P. ayeaye , and P. megacephalus have similar karyotypes, which are typical of the genus Pithecopus . In P. palliatus the NOR was detected in the pericentromeric region of pair 9p whereas in P. ayeaye and P. megacephalus we report cases of the multiple NOR sites in karyotypes. In P. ayeaye the NOR was detected in the pericentromeric region of pair 9p in both homologues and additional sites was detected in pairs 3q, 4p, and 8q, all confirmed by FISH experiments. Already in P. megacephalus the NOR sites were detected in pericentromeric region homologues of pair 8q and additionally in one chromosome of pair 13q. A comparative overview of all the Pithecopus karyotypes analyzed up to now indicates the recurrence of the NOR-bearing chromosome pairs and the position of the NORs sites on these chromosomes. We hypothesized that this feature is a result of a polymorphic condition present in the common ancestor of Pithecopus . In such case, the lineages derived from polymorphic ancestor have reached fixation independently after divergence of lineages, resulting in a high level of homoplasy observed in this marker. Our findings help to fill the gaps in the understanding of the karyotype of the genus Pithecopus and reinforce the role of the evolutionary dynamics of the rDNA genes in karyotype diversification in this group.

          Related collections

          Most cited references 39

          • Record: found
          • Abstract: not found
          • Article: not found

          Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method

            Bookmark
            • Record: found
            • Abstract: not found
            • Article: not found

            Chromosome banding in Amphibia

             M G Schmid (1978)
              Bookmark
              • Record: found
              • Abstract: not found
              • Article: not found

              Phylogenetics, classification, and biogeography of the treefrogs (Amphibia: Anura: Arboranae)

                Bookmark

                Author and article information

                Contributors
                Journal
                Comp Cytogenet
                Comp Cytogenet
                8
                urn:lsid:arphahub.com:pub:A71ED5FC-60ED-5DA3-AC8E-F6D2BB5B3573
                urn:lsid:zoobank.org:pub:C8FA3ADA-5585-4F26-9215-A520EE683979
                Comparative Cytogenetics
                Pensoft Publishers
                1993-0771
                1993-078X
                2019
                21 October 2019
                : 13
                : 4
                : 325-338
                Affiliations
                [1 ] Programa de Pós-Graduação em Genética (PPG-GEN). Departamento de Genética, Universidade Federal do Paraná (UFPR), Setor de Biológicas, Av. Coronel Francisco H. Dos Santos, 100, 81531-980, Curitiba, PR, Brazil Universidade Federal do Paraná Curitiba Brazil
                [2 ] Departamento de Biologia Estrutural and Funcional, Universidade Estadual de Campinas (UNICAMP), Cidade Universitária, 13083-863, Campinas, SP, Brazil Universidade Estadual de Campinas Campinas Brazil
                Author notes
                Corresponding author: Daniel Pacheco Bruschi ( danielpachecobruschi@ 123456gmail.com )

                Academic editor: L. Kupriyanova

                Article
                37687
                10.3897/CompCytogen.v13i4.37687
                6817578
                Joana Moura Gama, Camilla Borges Gazolla, Deborah Yasmin de Souza, Shirlei Maria Recco-Pimentel, Daniel Pacheco Bruschi

                This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                Funding
                Funded by: Fundação de Amparo à Pesquisa do Estado de São Paulo 501100001807 http://doi.org/10.13039/501100001807
                Categories
                Research Article
                Amphibia
                Anura
                Chordata
                Genetics
                Molecular Cytogenetics
                South America

                chromosomal evolution, pithecopus

                Comments

                Comment on this article