Cohesive Properties of Terminally Differentiated Keratinocytes

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Abstract

The stratum corneum can be dissociated into single squames by several extraction methods: mechanically, with organic solvents, and with detergents. We have performed studies of reaggregation from single squames prepared by these methods. Mechanically dispersed corneocytes recombined into a lamellar-like structure closely resembling intact stratum corneum. Squames obtained by dissociation in ether formed a multilayered structure which differed from the intact tissue in the collapse of the bilayered lamellar structure into a single lucent band with osmiophilic deposits. Squames obtained after extraction with detergent failed to reaggregate.

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Journal ID (publisher-id): PAT

Journal ID (nlm-ta): Pathobiology

Journal ID (doi): 10.1159/issn.1015-2008

Title: Pathobiology

Publisher: S. Karger AG

ISSN (Print): 1015-2008

ISSN (Electronic): 1423-0291

Publication date Subscription-year: 1989

Publication date (Print): 1989

Publication date (Electronic): 07 October 2008

Volume: 57

Issue: 2

Pages: 60-66

Affiliations

Departments of ^aDermatology, ^bPathology, ^cMicrobiology, and ^dHuman Biological Chemistry and Genetics, and ^cShriner Burns Institute, University of Texas Medical Branch, Galveston, Tex., USA

Article

Publisher ID: 163509 Other ID: Expl Cell Biol 1989;57:60–66

DOI: 10.1159/000163509

SO-VID: 58ae14df-1494-4c47-8678-a351b2e99237

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Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

History

Date received : 22 August 1988

Date accepted : 17 October 1988

Page count

Pages: 7

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Cohesive Properties of Terminally Differentiated Keratinocytes

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Karger: Oncology

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