8
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Quantitative trait loci in pepper control the effective population size of two RNA viruses at inoculation.

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Infection of plants by viruses is a complex process involving several steps: inoculation into plant cells, replication in inoculated cells and plant colonization. The success of the different steps depends, in part, on the viral effective population size (Ne), defined as the number of individuals passing their genes to the next generation. During infection, the virus population will undergo bottlenecks, leading to drastic reductions in Ne and, potentially, to the loss of the fittest variants. Therefore, it is crucial to better understand how plants affect Ne. We aimed to (i) identify the plant genetic factors controlling Ne during inoculation, (ii) understand the mechanisms used by the plant to control Ne and (iii) compare these genetic factors with the genes controlling plant resistance to viruses. Ne was measured in a doubled-haploid population of Capsicum annuum. Plants were inoculated with either a Potato virus Y (PVY) construct expressing the green fluorescent protein or a necrotic variant of Cucumber mosaic virus (CMV). Newas assessed by counting the number of primary infection foci on cotyledons for PVY or the number of necrotic local lesions on leaves for CMV. The number of foci and lesions was correlated (r=0.57) and showed a high heritability (h2=0.93 for PVY and h2=0.98 for CMV). The Ne of the two viruses was controlled by both common quantitative trait loci (QTLs) and virus-specific QTLs, indicating the contribution of general and specific mechanisms. The PVY-specific QTL colocalizes with a QTL that reduces PVY accumulation and the capacity to break down a major-effect resistance gene.

          Related collections

          Author and article information

          Journal
          J. Gen. Virol.
          The Journal of general virology
          Microbiology Society
          1465-2099
          0022-1317
          Jul 2017
          : 98
          : 7
          Affiliations
          [1 ] 1​INRA, UR1052 GAFL, Unité de Génétique et Amélioration des Fruits et Légumes, Domaine St Maurice - 67 Allée des Chênes, CS 60094, F-84143 Montfavet Cedex, France 2​INRA, UR407 PV, Unité de Pathologie Végétale, Domaine St Maurice - 67 Allée des Chênes, CS 60094, F-84143 Montfavet Cedex, France.
          [2 ] 3​INRIA, Biocore Team, F-06902 Sophia Antipolis, France 4​INRA, Université Nice Sophia Antipolis, CNRS, UMR 1355-7254 Institut Sophia Agrobiotech, Sophia Antipolis, France 2​INRA, UR407 PV, Unité de Pathologie Végétale, Domaine St Maurice - 67 Allée des Chênes, CS 60094, F-84143 Montfavet Cedex, France.
          [3 ] 2​INRA, UR407 PV, Unité de Pathologie Végétale, Domaine St Maurice - 67 Allée des Chênes, CS 60094, F-84143 Montfavet Cedex, France.
          [4 ] 1​INRA, UR1052 GAFL, Unité de Génétique et Amélioration des Fruits et Légumes, Domaine St Maurice - 67 Allée des Chênes, CS 60094, F-84143 Montfavet Cedex, France.
          [5 ] 3​INRIA, Biocore Team, F-06902 Sophia Antipolis, France 4​INRA, Université Nice Sophia Antipolis, CNRS, UMR 1355-7254 Institut Sophia Agrobiotech, Sophia Antipolis, France.
          [6 ] 3​INRIA, Biocore Team, F-06902 Sophia Antipolis, France.
          [7 ] 5​INRA, UMR 1065 Santé et Agroécologie du Vignoble, BP 81, 33883 Villenave d'Ornon cedex, France.
          Article
          10.1099/jgv.0.000835
          28691663
          58eb03cd-6a69-47bb-8c5c-e1b32b141533
          History

          Comments

          Comment on this article