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      CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture

      1 , 1 , 1 , 1 , 1 , 2

      Annual Review of Plant Biology

      Annual Reviews

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          Abstract

          Enhanced agricultural production through innovative breeding technology is urgently needed to increase access to nutritious foods worldwide. Recent advances in CRISPR/Cas genome editing enable efficient targeted modification in most crops, thus promising to accelerate crop improvement. Here, we review advances in CRISPR/Cas9 and its variants and examine their applications in plant genome editing and related manipulations. We highlight base-editing tools that enable targeted nucleotide substitutions and describe the various delivery systems, particularly DNA-free methods, that have linked genome editing with crop breeding. We summarize the applications of genome editing for trait improvement, development of techniques for fine-tuning gene regulation, strategies for breeding virus resistance, and the use of high-throughput mutant libraries. We outline future perspectives for genome editing in plant synthetic biology and domestication, advances in delivery systems, editing specificity, homology-directed repair, and gene drives. Finally, we discuss the challenges and opportunities for precision plant breeding and its bright future in agriculture.

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          Most cited references 134

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          Targeting DNA double-strand breaks with TAL effector nucleases.

          Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the FokI endonuclease. Both native and custom TALE-nuclease fusions direct DNA double-strand breaks to specific, targeted sites.
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            Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9.

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              Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease.

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                Author and article information

                Journal
                Annual Review of Plant Biology
                Annu. Rev. Plant Biol.
                Annual Reviews
                1543-5008
                1545-2123
                April 29 2019
                April 29 2019
                : 70
                : 1
                : 667-697
                Affiliations
                [1 ]State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, China 100101;
                [2 ]University of Chinese Academy of Sciences, Beijing, China 100864
                Article
                10.1146/annurev-arplant-050718-100049
                © 2019

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