Blog
About

  • Record: found
  • Abstract: found
  • Article: not found

Biological identifications through DNA barcodes.

Proceedings of the Royal Society B: Biological Sciences

Sequence Analysis, DNA, Sequence Alignment, Polymerase Chain Reaction, Phylogeny, genetics, classification, Lepidoptera, Genetic Variation, Evolution, Molecular, chemistry, Electron Transport Complex IV, DNA, Mitochondrial, Animals

Read this article at

ScienceOpenPublisherPMC
Bookmark
      There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

      Abstract

      Although much biological research depends upon species diagnoses, taxonomic expertise is collapsing. We are convinced that the sole prospect for a sustainable identification capability lies in the construction of systems that employ DNA sequences as taxon 'barcodes'. We establish that the mitochondrial gene cytochrome c oxidase I (COI) can serve as the core of a global bioidentification system for animals. First, we demonstrate that COI profiles, derived from the low-density sampling of higher taxonomic categories, ordinarily assign newly analysed taxa to the appropriate phylum or order. Second, we demonstrate that species-level assignments can be obtained by creating comprehensive COI profiles. A model COI profile, based upon the analysis of a single individual from each of 200 closely allied species of lepidopterans, was 100% successful in correctly identifying subsequent specimens. When fully developed, a COI identification system will provide a reliable, cost-effective and accessible solution to the current problem of species identification. Its assembly will also generate important new insights into the diversification of life and the rules of molecular evolution.

      Related collections

      Author and article information

      Journal
      10.1098/rspb.2002.2218
      1691236
      12614582

      Comments

      Comment on this article