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      A monoclonal antibody specifically modulates dihydropyridine-sensitive calcium current in BC3H1 myocytes.

      1 ,
      Molecular pharmacology

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          Abstract

          The nonfusing muscle cell line BC3H1 expresses functional Na and Ca channels similar to those found in skeletal muscle (1). We have utilized a monoclonal antibody that cross-reacts with a 45-50-kDa glycoprotein in differentiated BC3H1 myocytes but not in cardiac or neuronal cells. This antibody, MAb1223, specifically modulates 45Ca influx and slowly activating, dihydropyridine-sensitive Ca currents when added to BC3H1 myocytes. Low-threshold, dihydropyridine-insensitive Ca currents and Na and K currents are unaffected. MAb1223 action is voltage dependent. At very negative holding potentials, similar to those of the resting cell, MAb1223 increases slow Ca current without significant changes in the voltage dependence of activation. At more positive potentials, at which channel opening probability is reduced by inactivation, exposure to MAb1223 reduces current. Agonist and antagonist dihydropyridines modulate the action of MAb1223 on the slow Ca channel in a manner that suggests that their respective binding sites may interact directly with the channel.

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          Author and article information

          Journal
          Mol. Pharmacol.
          Molecular pharmacology
          0026-895X
          0026-895X
          Oct 1988
          : 34
          : 4
          Affiliations
          [1 ] Department of Physiology and Molecular Biophysics, Baylor College of Medicine, Houston, Texas 77030.
          Article
          2845251
          5958d6fd-e6eb-4cd3-a919-fce49d1d9660
          History

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