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      Localization by photoaffinity labeling of natriuretic peptide receptor-A binding domain.

      Biochemistry
      Affinity Labels, metabolism, Amino Acid Sequence, Animals, Binding Sites, Blotting, Western, Cattle, Chymotrypsin, Cross-Linking Reagents, Cyanogen Bromide, Electrophoresis, Polyacrylamide Gel, Glycosylation, Guanylate Cyclase, chemistry, Humans, Ligands, Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase, Molecular Sequence Data, Natriuretic Agents, Peptide Fragments, Protein Binding, Rats, Receptors, Atrial Natriuretic Factor, Serine Endopeptidases, Trypsin, Zona Glomerulosa

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          Abstract

          A portion of the ligand binding domain for atrial natriuretic peptide (ANP) was identified as an affinity cross-linked proteolytic fragment of bovine adrenal natriuretic peptide receptor type-A (NPR-A). Affinity purified NPR-A was UV-cross-linked to the amino terminus of 125I-[Tyr2] rat ANP-(2-27). A chymotryptic fragment of the affinity labeled NPR-A was isolated by chromatography and electrophoresis. This fragment yielded a major microsequence corresponding to a region from Met173 to Phe188 of the receptor extracellular domain and containing one N-glycosylation site at Asn180. Bovine NPR-A receptor was then cross-linked to the carboxy terminus of the highly efficient photoaffinity derivative 125I-[Tyr18,Bpa27] rat ANP(1-27). Proteolysis of the affinity labeled NPR-A with cyanogen bromide and trypsin produced radiolabeled and glycosylated fragments of size 15 and 9 kDa, respectively, which contained the epitope Ile181-Phe188 (CS328) and which were detectable by immunoprecipitation with a monospecific polyclonal antibody against CS328. Proteolysis with cyanogen bromide followed by Glu-C produced a shorter photolabeled 6 kDa fragment which was not immunoprecipitable by anti-CS328 antibody and which was not glycosylated. The results lead to the identification of the short segment Asp191-Arg198 as the site of covalent binding of [Tyr18,Bpa27] rat ANP(1-27). This hydrophilic region is adjacent to the epitope Ile181-Phe188 and to the glycosylation site Asn180. It displays the species variability and the high surface probability expected for a portion of the binding domain of NPR-A in contact with ANP.

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