+1 Recommend
0 collections
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Endothelial and beta cell composite aggregates for improved function of a bioartificial pancreas encapsulation device

      Read this article at

          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.



          Encapsulation of pancreatic islets or beta cells is a promising strategy for treatment of type 1 diabetes by providing an immune isolated environment and allowing for transplantation in a different location than the liver. However, islets used for encapsulation often show lower functionality due to the damaging of islet endothelial cells during the isolation procedure. Factors produced by endothelial cells have great impact on beta cell insulin secretion. Therefore, mutual signaling between endothelial cells and beta cells should be considered for the development of encapsulation systems to achieve high insulin secretion and maintain beta cell viability. Here, we investigate whether co-culture of beta cells with endothelial cells could improve beta cell function within encapsulation devices.

          Materials and methods:

          Mouse insulinoma MIN6 cells and human umbilical vein endothelial cells were used for creating composite aggregates on agarose microwell platform. The composite aggregates were encapsulated within flat poly(ether sulfone)/polyvinylpyrrolidone device. Their functionality was assessed by glucose-induced insulin secretion test and compared to non-encapsulated free-floating aggregates.


          We created composite aggregates of 80–100 µm in diameter, closely mimicking pancreatic islets. Upon glucose stimulation, their insulin secretion is improved in comparison to aggregates consisting of only MIN6 cells. Moreover, the composite aggregates encapsulated within a device secrete more insulin than aggregates consisting of only MIN6 cells.


          Composite aggregates of MIN6 cells with human umbilical vein endothelial cells have improved insulin secretion in comparison to MIN6 aggregates showing that the interaction of beta cell and endothelial cell is crucial for a functional encapsulation system.

          Related collections

          Most cited references 35

          • Record: found
          • Abstract: found
          • Article: not found

          The vascular basement membrane: a niche for insulin gene expression and Beta cell proliferation.

          Endocrine pancreatic beta cells require endothelial signals for their differentiation and function. However, the molecular basis for such signals remains unknown. Here, we show that beta cells, in contrast to the exocrine pancreatic cells, do not form a basement membrane. Instead, by using VEGF-A, they attract endothelial cells, which form capillaries with a vascular basement membrane next to the beta cells. We have identified laminins, among other vascular basement membrane proteins, as endothelial signals, which promote insulin gene expression and proliferation in beta cells. We further demonstrate that beta1-integrin is required for the beta cell response to the laminins. The proposed mechanism explains why beta cells must interact with endothelial cells, and it may apply to other cellular processes in which endothelial signals are required.
            • Record: found
            • Abstract: found
            • Article: not found

            Pancreatic islet production of vascular endothelial growth factor--a is essential for islet vascularization, revascularization, and function.

            To investigate molecular mechanisms controlling islet vascularization and revascularization after transplantation, we examined pancreatic expression of three families of angiogenic factors and their receptors in differentiating endocrine cells and adult islets. Using intravital lectin labeling, we demonstrated that development of islet microvasculature and establishment of islet blood flow occur concomitantly with islet morphogenesis. Our genetic data indicate that vascular endothelial growth factor (VEGF)-A is a major regulator of islet vascularization and revascularization of transplanted islets. In spite of normal pancreatic insulin content and beta-cell mass, mice with beta-cell-reduced VEGF-A expression had impaired glucose-stimulated insulin secretion. By vascular or diffusion delivery of beta-cell secretagogues to islets, we showed that reduced insulin output is not a result of beta-cell dysfunction but rather caused by vascular alterations in islets. Taken together, our data indicate that the microvasculature plays an integral role in islet function. Factors modulating VEGF-A expression may influence islet vascularity and, consequently, the amount of insulin delivered into the systemic circulation.
              • Record: found
              • Abstract: found
              • Article: not found

              Morphological evidence for pancreatic polarity of beta-cell within islets of Langerhans.

              Reconstructions from serial paraffin sections were used to define the relationships of the pancreatic endocrine cells and the microvasculature of the islets of Langerhans of the rat. We have reported that the afferent vessel to an islet, an arteriole, enters the islet directly in the beta-cell core. Immediately after entering the islet, this arteriole branches into capillaries. Herein, we report that beta-cells have two capillary faces. When viewed in cross section, 8-10 beta-cells form a tubelike structure around a central capillary. The outer side of each beta-cell also abuts a capillary. A clear polarity of secretory granules was seen on the ultrastructural level when the beta-cells were partially degranulated by prior in vivo treatment with glyburide. Of the remaining granules, 75.7 +/- 1.7% were accumulated in the apical portion of the cell, even though this portion was only 50.4 +/- 3.2% of the cytoplasm. beta-Cells around a particular cross-sectioned capillary shared the same orientation of granular accumulation. These findings provide morphological evidence of in situ beta-cell polarity that could be an anatomical basis for functional compartmentalization.

                Author and article information

                [1 ]Bioartificial Organs, Biomaterials Science and Technology, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands
                [2 ]Biomedical Materials Group and Institute of Pharmacy, Martin Luther University of Halle-Wittenberg, Halle, Germany
                [3 ]Interdisciplinary Centre of Material Sciences, Martin Luther University of Halle-Wittenberg, Halle, Germany
                Author notes
                Dimitrios Stamatialis, Bioartificial Organs, Biomaterials Science and Technology, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands. Email: d.stamatialis@
                Int J Artif Organs
                Int J Artif Organs
                The International Journal of Artificial Organs
                SAGE Publications (Sage UK: London, England )
                20 February 2018
                March 2018
                : 41
                : 3
                : 152-159
                © The Author(s) 2018

                This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License ( which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (

                Original research articles
                Artificial lung, ECMO and respiratory support


                Comment on this article