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      Isolation of Saccharomyces Cerevisiae from Pineapple and Orange and Study of Metal’s Effectiveness on Ethanol Production

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          Abstract

          In view of the anticipated shortage of the traditional supplies of fossil fuels, there is a great deal of interest in the production of ethanol as an alternative biofuel in recent years. The main objective of this research work was to isolate and characterize stress tolerant, high potential ethanol producing yeast strains from various fruit peel. Two yeast isolates from pineapple (Pa) and orange (Or) have been isolated, characterized on the basis of morphological and physic-chemical characters and optimized on ethanol producing capability using sugarcane molasses as substrate. Ethanol production percentage was estimated by Conway method. Isolates were thermotolerant, pH tolerant, ethanol tolerant as well as osmotolerant. They were resistant to Chloramphenicol (30 μg/disc) and Nalidixic acid (30 μg/disc). The isolates showed no killer toxin activity against E. coli. The highest production capacity of the yeasts was found to be 7.39% and 5.02% for Pa and Or, respectively, at pH 5.0, 30 °C temperature in media with an initial reducing sugar concentration of 6.5% for Pa and 5.5% for Or (shaking). Addition of metal ions increased the rate of ethanol production highest to 10.61% by KH 2PO 4. This study revealed that indigenous yeast isolates could be used to benefit the fuel demand and industrial alcohol industries.

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          Most cited references 89

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          Proxy global assessment of land degradation

           Z-G Bai,  D. Dent,  M-L Olsson (2008)
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            Ethanol-diesel fuel blends -- a review.

            Ethanol is an attractive alternative fuel because it is a renewable bio-based resource and it is oxygenated, thereby providing the potential to reduce particulate emissions in compression-ignition engines. In this review the properties and specifications of ethanol blended with diesel fuel are discussed. Special emphasis is placed on the factors critical to the potential commercial use of these blends. These factors include blend properties such as stability, viscosity and lubricity, safety and materials compatibility. The effect of the fuel on engine performance, durability and emissions is also considered. The formulation of additives to correct certain key properties and maintain blend stability is suggested as a critical factor in ensuring fuel compatibility with engines. However, maintaining vehicle safety with these blends may entail fuel tank modifications. Further work is required in specifying acceptable fuel characteristics, confirming the long-term effects on engine durability, and ensuring safety in handling and storing ethanol-diesel blends.
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              Characterization of alcohol-induced filamentous growth in Saccharomyces cerevisiae.

              Diploid cells of the budding yeast Saccharomyces cerevisiae starved for nitrogen differentiate into a filamentous growth form. Poor carbon sources such as starches can also stimulate filamentation, whereas haploid cells undergo a similar invasive growth response in rich medium. Previous work has demonstrated a role for various alcohols, by-products of amino acid metabolism, in altering cellular morphology. We found that several alcohols, notably isoamyl alcohol and 1-butanol, stimulate filamentous growth in haploid cells in which this differentiation is normally repressed. Butanol also induces cell elongation and changes in budding pattern, leading to a pseudohyphal morphology, even in liquid medium. The filamentous colony morphology and cell elongation require elements of the pheromone-responsive MAPK cascade and TEC1, whereas components of the nutrient-sensing machinery, such as MEP2, GPA2, and GPR1, do not affect this phenomenon. A screen for 1-butanol-insensitive mutants identified additional proteins that regulate polarized growth (BUD8, BEM1, BEM4, and FIG1), mitochondrial function (MSM1, MRP21, and HMI1), and a transcriptional regulator (CHD1). Furthermore, we have also found that ethanol stimulates hyperfilamentation in diploid cells, again in a MAPK-dependent manner. Together, these results suggest that yeast may sense a combination of nutrient limitation and metabolic by-products to regulate differentiation.
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                Author and article information

                Journal
                Eur J Microbiol Immunol (Bp)
                Eur J Microbiol Immunol (Bp)
                EUJMI
                European Journal of Microbiology & Immunology
                Akadémiai Kiadó (Budapest )
                2062-509X
                2062-8633
                27 February 2017
                March 2017
                : 7
                : 1
                : 76-91
                Affiliations
                Department of Mathematics and Natural Sciences, BRAC University , Bangladesh
                Author notes
                * Department of Mathematics and Natural Sciences, BRAC University, 66 Mohakhali, Dhaka-1212, Bangladesh; mmhossain@ 123456bracu.ac.bd

                + These authors contributed equally.

                Authors’ contribution

                A.N. carried out the collection and all experimental studies, analyzed and interpreted data, and drafted the thesis. S.S.R. carried out the collection and all experimental studies; analyzed and interpreted data; drafted, edited, finalized, and revised the article; and helped to coordinate the article to the publisher. M.M.H. and N.C. participated in designing, supervising, and coordinating the study and helped to revise the draft. All authors read and approved the final article.

                Competing interests

                The authors declare that they have no competing interests.

                Article
                10.1556/1886.2016.00035
                5372483
                © 2017, The Author(s)

                This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

                Page count
                Figures: 10, Tables: 14, Equations: 0, References: 62, Pages: 16
                Funding
                Funding sources: No external funding was received by the authors. All research was carried out as a partial fulfillment of Master’s dissertation and funded by Department of MNS, BRAC University and own expense of authors.
                Categories
                Original Article

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