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      Determination of caffeine and its metabolites by micellar electrokinetic capillary electrophoresis.

      1 ,
      Journal of chromatography. B, Biomedical sciences and applications
      Elsevier BV

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          Abstract

          The determination of caffeine and its analogues is important for a wide variety of analyses and is performed in an assortment of matrices ranging from food to clinical samples. While reversed-phase HPLC has become the standard analysis protocol in most laboratories, capillary electrophoresis has the advantages of higher separation efficiency and shorter separation time. The micellar capillary electrophoresis (MECC) separation of caffeine and its metabolites, theobromine, paraxanthine, theophylline and 1,3,7-trimethyluric acid was investigated using sodium dodecyl sulphate (SDS) as the micellar phase. The effects of pH, micelle concentration, buffer concentration, ionic strength, buffer salts, applied voltage and injection time were studied to select the optimum conditions for the determination of caffeine and its four analogues in drugs, foods and body fluids. Caffeine and its three analogues were resolved within 120 s with detection limits less than 1 microgram/ml. Samples could be analyzed utilizing direct injection with satisfactory resolution and reproducibility.

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          Author and article information

          Journal
          J Chromatogr B Biomed Sci Appl
          Journal of chromatography. B, Biomedical sciences and applications
          Elsevier BV
          1387-2273
          1387-2273
          Jan 24 1997
          : 688
          : 2
          Affiliations
          [1 ] Department of Chemistry, University of Kansas, Lawrence 66045, USA.
          Article
          10.1016/s0378-4347(96)00278-2
          9061464
          59c1e21c-1ebe-4727-b1e9-f042161b4227
          History

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