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      In-Use Evaluation of Peracetic Acid for High-Level Disinfection of Endoscopes.

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          Abstract

          Many high-level disinfectants have been used for disinfection of endoscopes such as 2% glutaraldehyde (GA), 0.55% ortho-phthalaldehyde (OPA), and peracetic acid (PAA). Both GA and OPA are widely used in disinfection of endoscopes and have been previously discussed, but there is little research on the practical use of PAA as an endoscope disinfectant. An experimental model of a flexible gastrointestinal endoscope being contaminated with 9 strains of microorganism was designed. After the cleaning and disinfecting procedure was completed, we evaluated the biocidal activity (850 ppm PAA, 2% GA, and 0.55% OPA) on our flexible gastrointestinal endoscope model. We also evaluated sterilization effectiveness of PAA on other bacteria, including some antibiotic-resistant bacteria (methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus, and Clostridium difficile). The residual bacterial colony count number of the PAA-disinfected endoscope was significantly lower than that of the GA- and OPA-disinfected endoscopes. The biocidal effect and efficiency of the endoscope disinfection by PAA appeared to be better than either the GA- or OPA-disinfected endoscope. PAA has demonstrated a good sterilization effect on other bacterial species; of particular note are common antibiotic-resistant bacteria such as methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus, and Clostridium difficile. The results of this study demonstrate that PAA is a fast and effective high-level disinfectant for use in the reprocessing of flexible endoscopes.

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          Author and article information

          Journal
          Gastroenterol Nurs
          Gastroenterology nursing : the official journal of the Society of Gastroenterology Nurses and Associates
          Ovid Technologies (Wolters Kluwer Health)
          1538-9766
          1042-895X
          April 14 2016
          : 39
          : 2
          Affiliations
          [1 ] Wu Chenjiao, MD, is Nurse-in-Charge, Digestive Endoscopy Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China. Zhang Hongyan, MD is Nurse-in-Charge, The First Teaching Hospital of Xinjiang Medical University Endoscopy Center, Wulumoqi, China. Gu Ging, MD is Associate Chief Nurse, Digestive Endoscopy Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China. Zhong Xiaoqi, MD is Nurse-in-Charge, Digestive Endoscopy Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China. Gu Liying, MD is Associate Chief Nurse, Digestive Endoscopy Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China. Fang Ying, MD is Associate Chief Nurse, Digestive Endoscopy Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
          Article
          00001610-201603000-00006
          10.1097/SGA.0000000000000192
          27070796
          59cbbb40-7434-472d-b2ff-be6df86ff651
          History

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