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      Software for the high-throughput collection of SAXS data using an enhanced Blu-Ice/ DCS control system

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          Abstract

          The Blu-Ice GUI and Distributed Control System ( DCS) developed in the Macromolecular Crystallography Group at the Stanford Synchrotron Radiation Laboratory has been optimized, extended and enhanced to suit the specific needs of the SAXS endstation at the SIBYLS beamline at the Advanced Light Source. The customizations reported here provide one potential route for other SAXS beamlines in need of robust and efficient beamline control software.

          Abstract

          Biological small-angle X-ray scattering (SAXS) provides powerful complementary data for macromolecular crystallography (MX) by defining shape, conformation and assembly in solution. Although SAXS is in principle the highest throughput technique for structural biology, data collection is limited in practice by current data collection software. Here the adaption of beamline control software, historically developed for MX beamlines, for the efficient operation and high-throughput data collection at synchrotron SAXS beamlines is reported. The Blu-Ice GUI and Distributed Control System ( DCS) developed in the Macromolecular Crystallography Group at the Stanford Synchrotron Radiation Laboratory has been optimized, extended and enhanced to suit the specific needs of the biological SAXS endstation at the SIBYLS beamline at the Advanced Light Source. The customizations reported here provide a potential route for other SAXS beamlines in need of robust and efficient beamline control software. As a great deal of effort and optimization has gone into crystallographic software, the adaption and extension of crystallographic software may prove to be a general strategy to provide advanced SAXS software for the synchrotron community. In this way effort can be put into optimizing features for SAXS rather than reproducing those that have already been successfully implemented for the crystallographic community.

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          Author and article information

          Journal
          J Synchrotron Radiat
          J. Synchrotron Rad.
          Journal of Synchrotron Radiation
          International Union of Crystallography
          0909-0495
          1600-5775
          01 November 2010
          03 September 2010
          03 September 2010
          : 17
          : Pt 6 ( publisher-idID: s100600 )
          : 774-781
          Affiliations
          [a ]Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
          [b ]Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
          [c ]Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158-2330, USA
          Author notes
          Correspondence e-mail: sclassen@ 123456lbl.gov
          Article
          ms5026 JSYRES S0909049510028566
          10.1107/S0909049510028566
          2964114
          20975223
          59d148db-846d-4b82-ba88-e0e37372d7a4
          © Scott Classen et al. 2010

          This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.

          History
          : 03 March 2010
          : 04 August 2010
          Categories
          Research Papers

          Radiology & Imaging
          control system,beamline,blu-ice,sibyls,dcs,gui,saxs,software
          Radiology & Imaging
          control system, beamline, blu-ice, sibyls, dcs, gui, saxs, software

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