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      Postmortem Changes of Glycolytic Metabolite Levels and Amino Acids in the Aqueous and Vitreous

      , ,

      Ophthalmic Research

      S. Karger AG

      Amino acids, Aqueous, Vitreous, Pig, Glycolysis, Postmortal changes

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          Abstract

          Glucose, lactate, pyruvate and amino acids were determined in the aqueous and vitreous of pig eyes directly after enucleation and after 6 and 12 h incubation of the globes at 22 °C in a moist chamber. In the aqueous, after 6 h and especially after 12 h of incubation the glucose and pyruvate levels were decreased, and lactate was elevated. The free amino acids, especially the essential amino acids, were reduced but showed insignificant elevation after 12 h. In the vitreous, the behavior of glucose, pyruvate and lactate was analogous to the aqueous, but the changes were slower. The free amino acids were increased by 1.5-fold and, after 12 h, by 3-fold, especially taurine, glycine and alanine. The essential amino acids were increased only after 12 h, by which time it may be supposed that considerable proteolysis has occurred.

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          Author and article information

          Journal
          ORE
          Ophthalmic Res
          10.1159/issn.0030-3747
          Ophthalmic Research
          S. Karger AG
          0030-3747
          1423-0259
          1976
          1976
          03 December 2009
          : 8
          : 4
          : 302-310
          Affiliations
          University Eye Clinic (Director: Prof. W.Staub), Marburg/Lahn
          Article
          264836 Ophthalmic Res 1976;8:302–310
          10.1159/000264836
          © 1976 S. Karger AG, Basel

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          Page count
          Pages: 9
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