Glucose, lactate, pyruvate and amino acids were determined in the aqueous and vitreous of pig eyes directly after enucleation and after 6 and 12 h incubation of the globes at 22 °C in a moist chamber. In the aqueous, after 6 h and especially after 12 h of incubation the glucose and pyruvate levels were decreased, and lactate was elevated. The free amino acids, especially the essential amino acids, were reduced but showed insignificant elevation after 12 h. In the vitreous, the behavior of glucose, pyruvate and lactate was analogous to the aqueous, but the changes were slower. The free amino acids were increased by 1.5-fold and, after 12 h, by 3-fold, especially taurine, glycine and alanine. The essential amino acids were increased only after 12 h, by which time it may be supposed that considerable proteolysis has occurred.