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      Determination of reference intervals for metabolic profile of Hanwoo cows at early, middle and late gestation periods

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          Abstract

          Background

          Metabolic profile was initially designed as a presymptomatic diagnostic aid based on statistical analyses of blood metabolites to provide an early warning of certain types of metabolic disorder. However, there is little metabolic profile data available about Korean Hanwoo cows. Therefore, this study aimed to determine the reference intervals of metabolic profile for Korean Hanwoo cows.

          Methods

          Healthy animals (2,205) were selected and divided into early (day 1 to 95), middle (day 96 to 190) and late (day 191 to 285) period according to their gestating period. Metabolic profile including total protein (TP), albumin (Alb), urea (UREA), glucose (Glu), total cholesterol (T-Cho), long-chain fatty acid (LCFA), aspartate aminotransferase (AST), gamma-glutamyl transpeptidase (GGT), creatinine (Crea), calcium (Ca), inorganic phosphorous (iP) and magnesium (Mg) were analyzed using a TBA-40FR automatic biochemical analyzer. The data of Korean Hanwoo cows were then compared to those of the Japanese Wagyu cows.

          Results

          Most of the data of the Korean Hanwoo cows were relatively higher than those of Japanese Wagyu cows, with the exception of Glu and GGT. This may indicate that the nutritional level of feed for the Korean Hanwoo cows was higher than that of the Japanese Wagyu cows because of the different feeding system. In particular, relatively higher levels of UREA and LCFA were observed in the Korean Hanwoo cows, and this may also contribute to the low reproduction efficiency.

          Conclusions

          These findings may provide some theoretical basis for understanding the reproductive and feeding situation of Korean Hanwoo cows.

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          Most cited references14

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          The use of a metabolic profile test in dairy herds.

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            Change of milk yield with clinical diseases for a high producing dairy herd.

            Changes in milk production associated with occurrence of clinical diseases (dystocia, stillbirth, twin births, milk fever, retained placenta, displaced abomasum, limping due to foot lesions, metritis, ketosis, and mastitis) were investigated. Data were collected daily on 388 lactation. Stepwise least squares regression was used to evaluate existence of associations between diseases and six yield measures that characterized milk production in the first 119 d postpartum. Logistic regression was used to investigate whether milk yield 1 to 5 d in milk might be of use to detect cows with early postpartum metritis (less than 21 d after calving). Lower milk production to 5 d postpartum was associated with an increased risk of early postpartum metritis in the logistic regression model. Yield to 5 and to 21 d postpartum was lower in cases of stillbirth, retained placenta, and early postpartum metritis. Yield from 22 to 49 d postpartum remained lower in cows diagnosed with early postpartum metritis. Milk yield losses occurred during diagnosis and treatment of displaced abomasum and mastitis. Ketosis was associated with yield losses prior to and at treatment. Ketosis to 21 d in milk was also associated with lower production after treatment. Limping diagnosed in the first 49 d postpartum coincided with higher yield to 5 d, to 21 d, and after 49 d postpartum.
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              Blood metabolic profiles: their use and relation to nutritional status of dairy cows.

              Profiles of blood metabolites have been used widely to identify problem herds and to indicate dietary causes of disease or low production. Related herd survey studies and designed experiments are reviewed. An initial study of profiles and estimated nutrient intake of five "normal" and four "problem" herds was conducted between 1972 and 1974 to establish baseline values for blood metabolites. Profiles of blood metabolites for additional problem herds were obtained during 1974 through 1977. Variation due to herd of origin, production, stage of lactation, and season of year are important causes of variation in concentrations of metabolites in jugular blood of dairy cows. They need to be considered carefully in interpreting profiles of blood metabolites. Several relationships between dietary intake of nutrients and concentrations of associated components in jugular blood are significant. Concentrations of metabolites are of almost no practical use for individual cows because of extreme variations in diet required to generate abnormal concentrations of blood metabolites. Averages of glucose of blood and nitrogen in urea on seven to ten cows, adjusted for the factors mentioned above, have limited practical value in detecting inadequate energy and protein intake, respectively.
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                Author and article information

                Contributors
                win_2008@hanmail.net
                cagewang@163.com
                foodleeking@gmail.com
                renevega10@yahoo.com
                cagewang@163.com
                cyjcow@snu.ac.kr
                hglee66@konkuk.ac.kr
                Journal
                J Anim Sci Biotechnol
                J Anim Sci Biotechnol
                Journal of Animal Science and Biotechnology
                BioMed Central (London )
                1674-9782
                2049-1891
                18 March 2015
                18 March 2015
                2015
                : 6
                : 1
                : 9
                Affiliations
                [ ]Laboratory of Animal Cell Biotechnology, Department of Agricultural Biotechnology, Seoul National University, Shinlim-dong, Kwanak-gu Seoul, 151-742 South Korea
                [ ]College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Nan-guan District Changchun, 130118 People’s Republic of China
                [ ]Key Laboratory of Animal Nutrition and Feed Science, Jilin Province, Jilin Agricultural University, 2888 Xincheng Street, Nan-guan District Changchun, 130118 People’ Republic of China
                [ ]Department of Animal Science and Technology, College of Animal Bioscience & Technology, Konkuk University, 120 Neungdong-ro, Gwangjin-gu Seoul, 143-701 South Korea
                [ ]Animal & Dairy Sciences Cluster, College of Agriculture, University of the Philippines Los Bañose, Los Baños, 4031 Laguna Philippines
                Article
                9
                10.1186/s40104-015-0009-0
                4389702
                59e9cce2-ca18-4a09-921c-1f2fafa945ba
                © Piao et al.; licensee BioMed Central. 2015

                This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

                History
                : 19 June 2014
                : 5 March 2015
                Categories
                Research
                Custom metadata
                © The Author(s) 2015

                Animal science & Zoology
                hanwoo cows,metabolic profile,reference intervals,wagyu cows
                Animal science & Zoology
                hanwoo cows, metabolic profile, reference intervals, wagyu cows

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