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      Changes in lung-specific molecular expression during differentiation of hamster embryonic M3E3/C3 cell line.

      Biochemistry and cell biology = Biochimie et biologie cellulaire
      Animals, Bronchi, cytology, metabolism, Cell Differentiation, Cell Line, Cricetinae, Cytoplasmic Granules, ultrastructure, DNA-Binding Proteins, genetics, Embryo, Mammalian, Gene Expression Profiling, Gene Expression Regulation, Developmental, Hepatocyte Nuclear Factor 3-alpha, Mesocricetus, Nuclear Proteins, Proteins, Proteolipids, Pulmonary Surfactant-Associated Proteins, Pulmonary Surfactants, RNA, Messenger, Respiratory Mucosa, Reverse Transcriptase Polymerase Chain Reaction, Transcription Factors, Uteroglobin, Vitamin A, pharmacology

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          Abstract

          To clarify the differentiation mechanisms of bronchiolar epithelial cells, changes at the transcription level of epithelial cell-specific proteins were examined using M3E3/C3, a cell line derived from hamster fetal lung. During a 9-day incubation period with 24 microg/mL of retinol, the cells became attached to each other and formed large bud-like structures which could be detected with periodic acid-Schiff staining. During the incubation period, the mRNA level of surfactant-associated protein-B significantly increased 2.6- and 5.4-fold higher than cells incubated without retinol on days 3 and 9, respectively. The Clara cell-specific secretory protein mRNA level also increased and peaked at 5.1-fold (P < 0.05) on day 5 compared with control cells. In contrast, mRNA for surfactant-associated protein-C, an alveolar type II cell-specific protein, decreased. Moreover, the expression of the gene for hepatocyte nuclear factor 3alpha, a putative transactivating factor for lung-related genes, was up-regulated resulting in consistently higher levels (2.4- to 5.6-fold) compared with controls, while those for transmembrane-type mucin-1 and glyceraldehyde-3-phosphate dehydrogenase were constantly expressed during the incubation. The present study confirms that at the gene transcription level M3E3/C3 cells differentiate into Clara-like cells with mucus granules in the presence of retinol.

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