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      MISTURAS VITAMÍNICAS NA REGENERAÇÃO DO MARACUJAZEIRO AMARELO (Passiflora edulis f. flavicarpa Deg.) Translated title: VITAMINIC MIXTURES ON YELLOW PASSION FRUIT REGENERATION

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          Abstract

          Cotilédones obtidos a partir de sementes germinadas in vitro foram usados como explantes com o objetivo de avaliar o efeito de dois complexos vitamínicos e de duas concentrações de 6-benzilaminopurina (BAP) na organogênese direta do maracujá amarelo (Passiflora edulis f. flavicarpa Deg.). Empregou-se o meio Murashige & Skoog (1962) suplementado com as vitaminas MS ou B5 (vitaminas de GAMBORG et al., 1968) e 1 ou 2 mg.<img SRC="http:/img/fbpe/cr/v32n2/a09img01.gif"> -1 de BAP. Foram testados quatro tratamentos, sendo cada um constituído de 60 explantes. O experimento foi inteiramente casualizado, com dez repetições. Os dados, analisados pelo teste de Tukey, demonstraram que os meios de cultura suplementados com o complexo vitamínico B5 induziram gemas em mais de 70% dos explantes, obtendo-se até 8 brotos por explante, com um enraizamento superior a 90%. A análise de ploidia das plantas regeneradas indicou um número aparentemente normal de cromossomos típicos dessa espécie (2n=18)

          Translated abstract

          Cotyledons were used as explants in order to test the effect of different vitamin mixtures and BAP concentrations on yellow passion fruit (Passiflora edulis f. flavicarpa Deg.) in vitro direct organogenesis. They basal medium was constituted of MS salts, supplemented with 1 and 2mg.<img SRC="http:/img/fbpe/cr/v32n2/a09img01.gif"> -1 of BAP (6-benzilaminopurina). Vitamin mixtures MS and B5 were tested. Every assay was done with 60 explants. The data were analyzed by Tukey's test. The medium supplemented with B5 vitaminic complex induced an increase of the capacity of cotyledons to regenerate in the presence of both BAP concentrations, as well for the number of explants with buds as for the number of buds per explant. The analysis of ploidy level of regenerated plants showed a number of chromosomes apparently normal and typical for this specie (2n=18).

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          Most cited references20

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          Plant propagation by tissue culture, part 1: the technology

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            In vitro control of adventitious bud differentiation by inorganic medium components and silver thiosulfate in explants of Passiflora edulis F. flavicarpa

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              In Vitro Micropropagation of Passiflora edulis (Purple Passionfruit)

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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                cr
                Ciência Rural
                Cienc. Rural
                Universidade Federal de Santa Maria (Santa Maria )
                1678-4596
                April 2002
                : 32
                : 2
                : 237-241
                Affiliations
                [1 ] Universidade Estadual de Ponta Grossa
                [2 ] Universidade Federal do Paraná Brazil
                [3 ] UEPG
                Article
                S0103-84782002000200009
                10.1590/S0103-84782002000200009
                5a205f0b-5c38-4b8f-b0a1-a66cc34d42ae

                http://creativecommons.org/licenses/by/4.0/

                History
                Product

                SciELO Brazil

                Self URI (journal page): http://www.scielo.br/scielo.php?script=sci_serial&pid=0103-8478&lng=en
                Categories
                AGRONOMY

                Horticulture
                organogenesis,tissue culture,cytology,Passiflora edulis f. flavicarpa,organogênese,cultura de tecidos,citologia,Passiflora edulis f. flavicarpa Deg

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