Alice F. Versiani 1 , 6 , 8 , Estefânia M. N. Martins 2 , 6 , Lidia M. Andrade 3 , 6 , Laura Cox 1 , Glauco C. Pereira 4 , Edel F. Barbosa-Stancioli 1 , Mauricio L. Nogueira 5 , Luiz O. Ladeira 3 , 6 , Flávio G. da Fonseca , 1 , 7
9 July 2020
The Flaviviridae virus family was named after the Yellow-fever virus, and the latin term flavi means “of golden color”. Dengue, caused by Dengue virus (DENV), is one of the most important infectious diseases worldwide. A sensitive and differential diagnosis is crucial for patient management, especially due to the occurrence of serological cross-reactivity to other co-circulating flaviviruses. This became particularly important with the emergence of Zika virus (ZIKV) in areas were DENV seroprevalence was already high. We developed a sensitive and specific diagnostic test based on gold nanorods (GNR) functionalized with DENV proteins as nanosensors. These were able to detect as little as one picogram of anti-DENV monoclonal antibodies and highly diluted DENV-positive human sera. The nanosensors could differentiate DENV-positive sera from other flavivirus-infected patients, including ZIKV, and were even able to distinguish which DENV serotype infected individual patients. Readouts are obtained in ELISA-plate spectrophotometers without the need of specific devices.