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      Multielectrode Array (MEA) Assay for Profiling Electrophysiological Drug Effects in Human Stem Cell-Derived Cardiomyocytes.

      1
      Current protocols in toxicology
      Wiley
      cardiomyocyte, cardiotoxicity, multielectrode array, preclinical, proarrhythmia, stem cell

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          Abstract

          More relevant and reliable preclinical cardiotoxicity tests are required to improve drug safety and reduce the cost of drug development. Human stem cell-derived cardiomyocytes (hSC-CMs) provide a potential model for the development of superior assays for preclinical drug safety screening. One such hSC-CM assay that has shown significant potential for enabling more predictive drug cardiac risk assessment is the MEA assay. The Multi-electrode Array (MEA) assay is an electrophysiology-based technique that uses microelectrodes embedded in the culture surface of each well to measure fluctuations in extracellular field potential (FP) generated from spontaneously beating hSC-CMs. Perturbations to the recorded FP waveform can be used as an unbiased method of predicting the identity of ion channel(s) impacted on drug exposure. Here, a higher throughput MEA assay using hSC-CMs in 48-well MEA plates is described for profiling compound-induced effects on cardiomyocyte electrophysiology. Techniques for preparing hSC-CM monolayers in MEA plates and methods to contextualize MEA assay experimental results are also covered. © 2016 by John Wiley & Sons, Inc.

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          Author and article information

          Journal
          Curr Protoc Toxicol
          Current protocols in toxicology
          Wiley
          1934-9262
          1934-9254
          May 04 2016
          : 68
          Affiliations
          [1 ] GE Healthcare Life Sciences, Maynard Centre, Forest Farm, Whitchurch, Cardiff, United Kingdom.
          Article
          10.1002/cptx.2
          27145112
          5a46ace3-35c1-4ee2-83a1-3797cd3fc89f
          History

          multielectrode array,cardiomyocyte,cardiotoxicity,preclinical,proarrhythmia,stem cell

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