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      Development of Lateral Flow Assay Based on Size-Controlled Gold Nanoparticles for Detection of Hepatitis B Surface Antigen

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          Abstract

          In this study, we developed lateral flow assay (LFA) biosensors for the detection of hepatitis B surface antigens using well-controlled gold nanoparticles (AuNPs). To enhance colorimetric signals, a seeded growth method was used for the preparation of size-controlled AuNPs with a narrow size distribution. Different sizes of AuNPs in the range of 342–137.8 nm were conjugated with antibodies and then optimized for the efficient detection of LFA biosensors. The conjugation stability was investigated by UV-vis spectroscopy of AuNP dispersion at various pH values and concentrations of antibody. Based on optimized conjugation conditions, the use of 42.7 ± 0.8 nm AuNPs exhibited superior performance for the detection of LFAs relative to other sizes of AuNPs.

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          Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature survey.

          Geertruida Posthuma-Trumpie, Jakob Korf, Aart van Amerongen (2009)
          Lateral flow (immuno)assays are currently used for qualitative, semiquantitative and to some extent quantitative monitoring in resource-poor or non-laboratory environments. Applications include tests on pathogens, drugs, hormones and metabolites in biomedical, phytosanitary, veterinary, feed/food and environmental settings. We describe principles of current formats, applications, limitations and perspectives for quantitative monitoring. We illustrate the potentials and limitations of analysis with lateral flow (immuno)assays using a literature survey and a SWOT analysis (acronym for "strengths, weaknesses, opportunities, threats"). Articles referred to in this survey were searched for on MEDLINE, Scopus and in references of reviewed papers. Search terms included "immunochromatography", "sol particle immunoassay", "lateral flow immunoassay" and "dipstick assay".
          Article 0 comments Cited 388 times – based on 0 reviews     Review now
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            Kinetically controlled seeded growth synthesis of citrate-stabilized gold nanoparticles of up to 200 nm: size focusing versus Ostwald ripening.

            Neus Bastus, Joan Comenge, Victor Puntes (2011)
            Monodisperse citrate-stabilized gold nanoparticles with a uniform quasi-spherical shape of up to ∼200 nm and a narrow size distribution were synthesized following a kinetically controlled seeded growth strategy via the reduction of HAuCl(4) by sodium citrate. The inhibition of any secondary nucleation during homogeneous growth was controlled by adjusting the reaction conditions: temperature, gold precursor to seed particle concentration, and pH. This method presents improved results regarding the traditional Frens method in several aspects: (i) it produces particles of higher monodispersity; (ii) it allows better control of the gold nanoparticle size and size distribution; and (iii) it leads to higher concentrations. Gold nanoparticles synthesized following this method can be further functionalized with a wide variety of molecules, hence this method appears to be a promising candidate for application in the fields of biomedicine, photonics, and electronics, among others. © 2011 American Chemical Society
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              Lateral flow assays: Principles, designs and labels

              Elif Bahadır, Mustafa Sezgintürk (2016)
              Article 0 comments Cited 150 times – based on 0 reviews     Review now
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                Author and article information

                Contributors
                Huangxian Ju: Role: Academic Editor
                Journal
                Journal ID (nlm-ta): Sensors (Basel)
                Journal ID (iso-abbrev): Sensors (Basel)
                Journal ID (publisher-id): sensors
                Title: Sensors (Basel, Switzerland)
                Publisher: MDPI
                ISSN (Electronic): 1424-8220
                Publication date (Electronic): 16 December 2016
                Publication date Collection: December 2016
                Volume: 16
                Issue: 12
                Electronic Location Identifier: 2154
                Affiliations
                [1 ]Department of Chemical Engineering, Kangwon National University, Samcheok 25913, Korea; nyongtory91@ 123456gmail.com (D.S.K.); skychojja@ 123456gmail.com (S.B.H.)
                [2 ]Nanobio Application Team, National NanoFab Center (NNFC), Daejeon 34141, Korea; ytkim@ 123456nnfc.re.kr (Y.T.K.); mklee@ 123456nnfc.re.kr (M.-K.L.); sjlee@ 123456nnfc.re.kr (S.J.L.)
                [3 ]Department of Biological Engineering, Inha University, 100 Inha-ro, Nam-gu, Incheon 22212, Korea; anlfkrn2000@ 123456gmail.com (J.K.); bioheo6@ 123456gmail.com (N.S.H.)
                [4 ]Research and Development Center, Tomorrow & Solution (T&S), E19-604, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Korea; kbiset@ 123456gmail.com
                [5 ]Research Center 2, Eudipia Inc., Cheongju 361-951, Korea; i.s.kim@ 123456eudipia.com
                Author notes
                [* ]Correspondence: yunsuk.huh@ 123456inha.ac.kr (Y.S.H.); bgchoi@ 123456kangwon.ac.kr (B.G.C.); Tel.: +82-10-2781-3478 (B.G.C.)
                Article
                Publisher ID: sensors-16-02154
                DOI: 10.3390/s16122154
                PMC ID: 5191134
                PubMed ID: 27999291
                SO-VID: 5a5becf3-6939-4837-ab3f-7bd32edff88e
                Copyright © © 2016 by the authors; licensee MDPI, Basel, Switzerland.
                License:

                This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license ( http://creativecommons.org/licenses/by/4.0/).

                History
                Date received : 12 September 2016
                Date accepted : 12 December 2016
                Categories
                Subject: Article

                ScienceOpen disciplines: Biomedical engineering
                Keywords: gold,nanoparticle,hepatitis b surface antigen,lateral flow assay,conjugation
                Data availability:
                ScienceOpen disciplines: Biomedical engineering
                Keywords: gold, nanoparticle, hepatitis b surface antigen, lateral flow assay, conjugation

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