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      Molecular dissection of the response of a rice leucine-rich repeat receptor-like kinase (LRR-RLK) gene to abiotic stresses.

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          Abstract

          Leucine-rich repeat (LRR) receptor-like kinase (RLK) proteins play key roles in a variety of biological pathways. In a previous study, we analyzed the members of the rice LRR-RLK gene family using in silico analysis. A total of 23 LRR-RLK genes were selected based on the expression patterns of a genome-wide dataset of microarrays. The Oryza sativa gamma-ray induced LRR-RLK1 (OsGIRL1) gene was highly induced by gamma irradiation. Therefore, we studied its expression pattern in response to various different abiotic and phytohormone treatments. OsGIRL1 was induced on exposure to abiotic stresses such as salt, osmotic, and heat, salicylic acid (SA), and abscisic acid (ABA), but exhibited downregulation in response to jasmonic acid (JA) treatment. The OsGIRL1 protein was clearly localized at the plasma membrane. The truncated proteins harboring juxtamembrane and kinase domains (or only harboring a kinase domain) exhibited strong autophosphorylation. The biological function of OsGIRL1 was investigated via heterologous overexpression of this gene in Arabidopsis plants subjected to gamma-ray irradiation, salt stress, osmotic stress, and heat stress. A hypersensitive response was observed in response to salt stress and heat stress, whereas a hyposensitive response was observed in response to gamma-ray treatment and osmotic stress. These results provide critical insights into the molecular functions of the rice LRR-RLK genes as receptors of external signals.

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          Author and article information

          Journal
          J. Plant Physiol.
          Journal of plant physiology
          1618-1328
          0176-1617
          Nov 1 2014
          : 171
          : 17
          Affiliations
          [1 ] Plant Genomics Lab., Department of Applied Plant Sciences, Kangwon National University, Chuncheon 200-713, Republic of Korea.
          [2 ] Plant Genomics Lab., Department of Applied Plant Sciences, Kangwon National University, Chuncheon 200-713, Republic of Korea; Agriculture and Life Sciences Research Institute, Kangwon National University, Chuncheon 200-713, Republic of Korea.
          [3 ] Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 580-185, Republic of Korea.
          [4 ] Plant Genomics Lab., Department of Applied Plant Sciences, Kangwon National University, Chuncheon 200-713, Republic of Korea. Electronic address: csjang@kangwon.ac.kr.
          Article
          S0176-1617(14)00214-4
          10.1016/j.jplph.2014.08.002
          25173451
          5a70807a-c8db-43bc-ad41-bd5b255f3432
          Copyright © 2014 Elsevier GmbH. All rights reserved.
          History

          Abiotic stress,Gamma-ray,LRR-RLK,Molecular dissection,OsGIRL1

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