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The tetrodotoxin (TTX) contents of the Japanese fire-bellied newt, Cynops pyrrhogaster,
captive-reared from eggs to metamorphosed juveniles with a non-toxic diet for 70 weeks,
as well as wild-caught juvenile newts, were investigated using a high-resolution hydrophilic
interaction chromatography-LC-MS. TTX was detected in 0- to 22-week-old captive-reared
juvenile newts but was not detected (<15 ng/g) in the 36- to 70-week-old newts, while
significant levels of TTX (1.3-14 μg/g) were detected in the wild-caught juveniles.