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      Molecular memory of prior infections activates the CRISPR/Cas adaptive bacterial immunity system.

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          Abstract

          CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated genes) is a small RNA-based adaptive prokaryotic immunity system that functions by acquisition of short fragments of DNA (mainly from foreign invaders such as viruses and plasmids) and subsequent destruction of DNA with sequences matching acquired fragments. Some mutations in foreign DNA that affect the match prevent CRISPR/Cas defensive function. Here we show that matching sequences that are no longer able to elicit defense, still guide the CRISPR/Cas acquisition machinery to foreign DNA, thus making the spacer acquisition process adaptive and leading to restoration of CRISPR/Cas-mediated protection. We present evidence suggesting that after initial recognition of partially matching foreign DNA, the CRISPR/Cas acquisition machinery moves along the DNA molecule, occasionally selecting fragments to be incorporated into the CRISPR locus. Our results explain how adaptive CRISPR/Cas immunity becomes specifically directed towards foreign DNA, allowing bacteria to efficiently counter individual viral mutants that avoid CRISPR/Cas defense.

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          Author and article information

          Journal
          Nat Commun
          Nature communications
          Springer Science and Business Media LLC
          2041-1723
          2041-1723
          Jul 10 2012
          : 3
          Affiliations
          [1 ] Waksman Institute, Piscataway, New Jersey 08854, USA.
          Article
          ncomms1937
          10.1038/ncomms1937
          22781758
          5a7f0e07-f852-496a-83d4-0bc75fc6aa50
          History

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