0
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found

      The Suppressive Effect of Delta-1-Tetrahydrocannabinol on the Steroidogenic Activity of Rat Granulosa Cells in Culture

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          In previous study the major psychoactive ingredient of marihuana (Δ<sup>1</sup>-THC) has been shown to inhibit ovarian prostaglandin synthesis when administered to normally cycling rats in the early afternoon of proestrus. These results suggested a direct suppressive effect of the drug on the ovary. The purpose of this study was to evaluate the effect of Δ<sup>1</sup>-THC on steroidogenesis in granulosa cells (GC) in vitro. Incubation of GC with Δ<sup>1</sup>-THC (10–50 µ M) effectively inhibited LH-stimulatable progestin production. This suppressive effect was not abolished by washing the cells after 24 h of culture in the presence of Δ<sup>1</sup>-THC, indicating the irreversible nature of the blocking effect of Δ<sup>1</sup>-THC. By contrast, estradiol production following incubation of GC with testosterone (1 µg/ml) was not inhibited by similar concentrations of Δ<sup>1</sup>-THC, thus suggesting that △<sup>1</sup>-THC does not inhibit aromatase activity in GC. In addition, Δ<sup>1</sup>-THC was shown to inhibit cAMP production as well as <sup>125</sup>I-hCG binding capacity to GC. Administration of 8-Br-cAMP did not abolish the Δ<sup>1</sup>-THC-induced block, suggesting that the drug probably acts distal to the cAMP site of action.

          Related collections

          Author and article information

          Journal
          HRE
          Horm Res Paediatr
          10.1159/issn.1663-2818
          Hormone Research in Paediatrics
          S. Karger AG
          1663-2818
          1663-2826
          1984
          1984
          26 November 2008
          : 19
          : 1
          : 43-51
          Affiliations
          aTimsit Institute of Reproductive Endocrinology, Municipal Governmental Medical Center, University of Tel-Aviv, Israel; bDepartment of Hormone Research, The Weizmann Institute of Science, Rehovot, Israel
          Article
          179858 Horm Res 1984;19:43–51
          10.1159/000179858
          6319259
          © 1984 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 9
          Categories
          Paper

          Comments

          Comment on this article