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      Identifying Sialylation Linkages at the Glycopeptide Level by Glycosyltransferase Labeling Assisted Mass Spectrometry (GLAMS)

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          Abstract

          <p class="first" id="P4">Precise assignment of sialylation linkages at the glycopeptide level is of importance in bottom-up glycoproteomics and an indispensable step to understand the function of glycoproteins in pathogen–host interactions and cancer progression. Even though some efforts have been dedicated to the discrimination of <i>α</i>2,3/ <i>α</i>2,6-sialylated isomers, unambiguous identification of sialoglycopeptide isomers is still needed. Herein, we developed an innovative glycosyltransferase labeling assisted mass spectrometry (GLAMS) strategy. After specific enzymatic labeling, oxonium ions from higher-energy C-trap dissociation (HCD) fragmentation of <i>α</i>2,3-sailoglycopeptides then generate unique reporters to distinctly differentiate those of <i>α</i>2,6-sailoglycopeptide isomers. With this strategy, a total of 1236 linkage-specific sialoglycopeptides were successfully identified from 161 glycoproteins in human serum. </p><p id="P5"> <div class="figure-container so-text-align-c"> <img alt="" class="figure" src="/document_file/09ba6d51-204e-4c82-85f5-1ece22106aab/PubMedCentral/image/nihms-1651430-f0001.jpg"/> </div> </p>

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          Essentials of Glycobiology

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            Author and article information

            Contributors
            Journal
            Analytical Chemistry
            Anal. Chem.
            American Chemical Society (ACS)
            0003-2700
            1520-6882
            May 05 2020
            April 09 2020
            May 05 2020
            : 92
            : 9
            : 6297-6303
            Affiliations
            [1 ]Department of Chemistry, Georgia State University, Atlanta, Georgia 30303, United States
            [2 ]Georgia Cancer Center, Augusta University, Augusta, Georgia 30912, United States
            [3 ]Department of Biochemistry and Molecular Biology, Augusta University, Augusta, Georgia 30912, United States
            [4 ]Department of Cancer Biology and Blais Proteomics Center, Dana-Farber Cancer Institute, Boston, Massachusetts 02215, United States
            Article
            10.1021/acs.analchem.9b05068
            7750919
            32271005
            5b095edc-dc3a-4bf8-9ebe-8b97a358ae37
            © 2020

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