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Abstract
To control the quality of rhizoma Coptidis, a method based on ultra performance liquid
chromatography with photodiode array detector (UPLC-PAD) was developed for quantitative
analysis of five active alkaloids and chemical fingerprint analysis. In quantitative
analysis, the five alkaloids showed good regression (R > 0.9992) within test ranges
and the recovery of the method was in the range of 98.4-100.8%. The limit of detections
and quantifications for five alkaloids in PAD were less than 0.07 and 0.22 microg/ml,
respectively. In order to compare the UPLC fingerprints between rhizoma Coptidis from
different origins, the chemometrics procedures, including similarity analysis (SA),
hierarchical clustering analysis (HCA), principal component analysis (PCA) were applied
to classify the rhizoma Coptidis samples according to their cultivated origins. Consistent
results were obtained to show that rhizoma Coptidis samples could be successfully
grouped in accordance with the province of origin. Furthermore, five marker constituents
were screened out to be the main chemical marker, which could be applied to accurate
discrimination and quality control for rhizoma Coptidis by quantitative analysis.
This study revealed that UPLC-PAD method was simple, sensitive and reliable for quantitative
and chemical fingerprint analysis, moreover, for the quality evaluation and control
of rhizoma Coptidis.