Tear Potassium Contributes to Maintenance of Corneal Thickness

Abstract

Isolated rabbit corneas were bathed on their endothelial surfaces with normal Krebs bicarbonate Ringer solution, while the epithelial surfaces were bathed in a basic tear solution containing sodium and potassium. When bathed in basic tear solution alone, corneal swelling occurred at an average of 12 μm/h over a 3-hour period. Corneal swelling occurred at a rate of about 21 μm/h when the epithelial solution was switched from normal basic tear solution to an iso-osmotic K⁺-free basic tear solution. Corneal swelling then slowed, and in the final hour of a 3-hour exposure to K⁺-free tear solution, the corneas deswelled at about 10 μm/h. The data indicate that potassium is a necessary solute for the maintenance of normal corneal thickness. The results suggest that a lacrimal dysfunction that would cause a decrease in the potassium content of tears may influence corneal thickness and also suggest that the inclusion of potassium in artificial tears is important.