While most experts agree that cervical screening is effective, there remains controversy over the most appropriate screening interval. Annual screening is common in North America (even in women who have been screened several times previously), while 5-yearly screening is provided by some European countries. Here, we estimate the benefits of screening at different intervals and at different ages from a large population-based case–control study. Following the relaunch of the National Cervical Screening Programmes in the United Kingdom in 1988, we initiated a protocol to monitor its effectiveness. Originally under the auspices of the National Coordinating Network and more recently with the support of the National Screening Office, we have been collecting data on the screening histories of all women from self-selected Health Authorities and Health Boards (HAs) with newly diagnosed cancer and a small control of sample women without cervical cancer. We previously reported results on 5 years of screening in 1025 women, including 348 with cancer (Sasieni et al, 1996). The full data set now has over 2500 women with cancer. Here we analyse data on 1305 women diagnosed with stage 1B or worse cervical cancer between the ages of 20 and 69 years, and 2532 controls with a total of approximately 40 000 women-years of screening (35 000 since 1988). Approximately 90% of cases in this report were not included in the previous report. The rationale for restricting attention to frankly invasive cancer is that ideally these should all be prevented by screening, whereas microinvasive cancers are mostly screen-detected and have an extremely good cure rate. MATERIALS AND METHODS The methods have been described previously (Sasieni et al, 1996). The participating HAs have changed over the years due to boundary changes and changes in personnel. Areas that contributed data and the years for which they contributed are listed in the Acknowledgements. Data were collected by local coordinators as part of audit. Cases were identified from pathology laboratories and confirmed to have been resident in the HA at diagnosis. Data recorded included the date of diagnosis and, where possible, the stage and histology of the cancers. Age-matched controls, for each case, were identified from among women (not known to have had a hysterectomy) registered with a group practice (GP) in the same area. One control was selected from the same GP, the other from another GP in the same area. Screening histories (including the dates and results of all smears) were downloaded from the Exeter computer system and checked against information held by cytology laboratories. The Exeter system is used to run the screening programme and stores screening histories of all women registered with a GP. All smears in this study were prepared by conventional (as opposed to liquid-based) cytology and classified according to a version of the British Society for Clinical Cytology (BSCC) system (Johnson and Patnick, 2000). In all analyses, a case's date of diagnosis was used as a pseudo-date of diagnosis for her matched controls, and only smears taken prior to that date were considered. All smears in teenagers were excluded because they are not part of the screening programme and are likely to identify a high-risk group. Odds ratios and their confidence intervals (CIs) were estimated by conditional logistic regression (Breslow and Day, 1980). As cervical cancer is a rare disease, odds ratios are interpreted as relative risks (RRs) and are referred to as such. Age groups refer to the age at diagnosis, not the age at which the smear was taken. One measure of exposure is the time between the last ‘operationally’ negative smear and (pseudo-) diagnosis. An operationally negative smear is defined as a negative smear not preceded by an abnormal smear (borderline or worse) within the previous 12 months. A second measure of exposure is the time prior to diagnosis of the most recent adequate screening smear (regardless of result) ignoring all smears within 6 months of diagnosis. A screening smear is defined here to be one that was not preceded (at any time) by an abnormal smear. An adequate smear is one that was not classed as inadequate (for making a good cytological classification). To estimate the protection from frankly invasive cervical cancer by being screened once every 3 years, we calculate the mean of the RRs for 0.5–1.5, 1.5–2.5 and 2.5–3.5 years, and similarly for 5 years. For ‘time since last negative’, the first interval is 0–1.5 years and is weighted accordingly when calculating the mean. The proportion preventable is one minus the mean RR. RESULTS The database includes 2753 women with invasive cervical cancer diagnosed between 1990 and 2001 (91% between 1992 and 1998). Their age distribution (Table 1 Table 1 Age distribution of invasive, microinvasive and unknown stage cases from this audit compared to all UK cervical cancer registrations Stage Age group (years) 1A 1B+ Unknown Total Registrations for UK 1993–1997 (%) 6 years 4.1 3.6 Never 18.4 16.3 Total 2532 1305 Time measure from date of (pseudo-) diagnosis. ). Half of all cases had a smear within 3 months of diagnosis compared to just 5% 6–12 months prior to diagnosis. In fact, the 6-month exclusion (used in Table 4) is not long enough to take account of all ‘diagnostic smears’ – the relative proportions last screened 6–12 months, compared to 1–6 years, prediagnosis is greater in cases than controls (P=0.002). Another difficulty with identifying the last screening test is that once a woman has been treated for a cervical lesion, she could be put on indefinite annual follow-up. Our solution of censoring screening histories at the first abnormal smear is not ideal and it potentially introduces a small bias in favour of screening. Further, the fact that the RRs (in Table 4) are greater in the first time period than the second suggests that the 6-month exclusion is not quite sufficient and that these RRs should not be overinterpreted. Despite these caveats, we believe that this approach does provide reasonable estimates of the efficacy of 3- and 5-yearly (but not annual) screening (Table 5). In younger women, the risk of disease in those whose last smear was more than 5.5 years ago was greater than in those who had no smears (Table 3 and 4). This suggests that those who opt out of screening altogether are at a lower underlying risk of cervical cancer than those who are screened occasionally. Opportunistic screening of women seeking contraceptive advice and those attending STD clinics could account for such a trend. What then is the appropriate baseline for estimating the RRs? We have used those with no smears, but use of those with no recent smear would have made the estimated effect of screening considerably greater in young women. The RR in women aged 20–39 years, whose most recent operationally negative smear was 3.5–4.5 years ago, was 1.06 relative to those with no such smear (Table 3). However, relative to those whose most recent negative smear was more than 6.5 years ago, it is 0.45 (=1.06/2.37). In our opinion, such adjusted RRs are inappropriate: (ignoring the effect of screening) it is more likely that those who were last screened many years ago form a high-risk subgroup than that those who are never screened are at low risk. But this needs to be tested by a larger, more detailed study in young women in which risk factors for the acquisition and persistence of HPV infection are collected along with screening histories. POLICY CONSIDERATIONS Policy should be determined by balancing costs against benefits. Although there are fixed overheads, the main cost is proportional to the number of screening tests. Thus, 3-yearly screening will cost 60–66% more than 5-yearly screening. This is partly offset by not having to pay for the treatment of cancers prevented, but the financial saving is modest. The main benefits are in terms of cancers prevented and lives saved and the latter can be converted into years of life saved. There are also ‘negative benefits’ such as unnecessary treatment and anxiety caused by abnormal smears. Icelandic data show that the amount of low-grade disease (and hence, presumably, the number of women made anxious) is inversely proportional to the screening interval (Sigurdsson and Adalsteinsson, 2001). One can try to combine these factors in an overall measure of quality of life, but it is difficult to balance the low level of anxiety provoked in many women against the prevention of cancer in a few. It is also unclear whether giving one woman an extra 30 years of life is equivalent to giving an extra week to 1500 women. Our results clearly show that cytological screening is less effective at preventing frankly invasive cervical cancer in women under the age of 40 than it is in women aged over 40 years. They also suggest that cervical cancer develops more rapidly in young women so that the incidence rate of cervical cancer 3 years after a negative smears is the same as that in unscreened women. It is possible that although screening is not very effective at preventing cancer in young women, it saves lives through early diagnosis. Indeed, in this series of 747 staged cancers in women aged 20–39 years, 41% were microinvasive. Our results in young women differ from those reported by IARC (1986). That paper summarised the results from three cohort studies with a total of 148 cases aged under 35 years and found that the protection, relative to historical incidence rates, was similar to that seen in older women. Cases in that study were restricted to those with squamous cancer, but included stage 1A tumours (including any that were screen-detected). It is thus possible that differences in the design of the studies could explain the different results. The RR associated with various screening intervals estimated here will help formulate policy, but there will be other considerations such as the underlying age-specific incidence rates, the numbers of years of cancer-free life gained and the age-specific rates of cytological abnormalities. Our own recommendations are given in Table 7 Table 7 Provisional screening recommendations for the UK Age group (years) Frequency of cytological screening Under 25 Do not screen 25–49 3-yearly screening 50–64 5-yearly screening 65+ Only screen those who have not been screened since age 50 Note that these age groups are shifted by 5 years from those elsewhere in this paper to allow for the time from screening to cancer diagnosis in Tables 3–5. . Under the age of 25 years, invasive cancer is extremely rare, but cytological abnormalities are common (Department of Health, 2001). Although lesions treated in very young women may prevent cancers from developing many years later, the results of this paper would suggest that it is enough to begin screening around age 25 – lesions that are destined to progress will still be screen-detectable and those that would regress will no longer be a source of anxiety. Nationally, only 1.7% of cervical cancer in women aged 20–69 occur under the age of 25, corresponding to an incidence rate of 2.5 per 100 000 women-years. In our study, 26 out of the 34 women with cervical cancer aged 20–24 years had a previous (operationally) negative smear, suggesting that cytology is not very sensitive for these tumours. A review of the screening histories of the 13 women with stage 1B+ cervical cancer aged 20–24 indicates that six of these cases were symptomatic, of which five were stage 1B and the other was stage 3. In the UK, cervical cancer rates between the ages of 25 and 40 years are only slightly lower than in older women, so effective screening in this group is essential. However, most cancers still occur in older women, so resources also have to be allocated to ensure that a high proportion of women continue to be screened (albeit less frequently) at older ages.