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      Temporary storage solution for adipose derived mesenchymal stem cells

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          Abstract

          Background

          We have developed a simple lipoaspirate washing method using a coffee filter to eliminate liposuction noxious material before isolating adipose tissue derived mesenchymal stem cells (AT-MSCs), and used them in clinical trials. Before administration to patients, MSCs are usually suspended in physiologic saline. However, MSCs only survive for a limited time in physiologic saline. Therefore, alternative solution that can preserve MSC survival will be beneficial. Therefore, the purpose of this study was to compare the use of physiologic saline and Dulbecco’s modified Eagle’s Medium (DMEM) as temporary storage solution for our AT-MSCs.

          Methods

          We did viability assessments of AT MSCs after 0, 3, 6, 24, 48, 72, and 96 hours suspended in physiologic saline compared to DMEM, and stored at 4 ℃. Further proliferation capacities of the cells after various suspension times were assessed. All viability and proliferation capacity assessments were done in four replications. Differences between the various suspension time in terms of viability and proliferation capacity were compared and tested by ANOVA or Kruskal-Wallis test.

          Results

          Viability was >70% after 48 hours in physiologic saline and 24 hours in DMEM, which showed that physiologic saline was superior compared to DMEM. Increase in PDT began to be significant compared to initial PDT after 24 hours in both physiologic saline, and DMEM.

          Conclusions

          For our AT-MSCs, physiologic saline was superior to DMEM, and storage should not exceed 24 hours.

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          Author and article information

          Journal
          Stem Cell Investig
          Stem Cell Investig
          SCI
          Stem Cell Investigation
          AME Publishing Company
          2306-9759
          2313-0792
          07 June 2018
          2018
          : 5
          Affiliations
          [1 ]Faculty of Pharmacy, Andalas university , Padang, Indonesia;
          [2 ]Stem Cell Medical Technology Integrated Service Unit, Dr. Cipto Mangunkusumo General Hospital/Faculty of Medicine Universitas Indonesia , Jakarta, Indonesia;
          [3 ]Department of Histology, Faculty of Medicine Universitas Indonesia , Jakarta, Indonesia;
          [4 ]Stem Cell and Tissue Engineering Research Center, Indonesia Medical Education and Research Institute (IMERI), Faculty of Medicine Universitas Indonesia , Jakarta, Indonesia
          Author notes

          Contributions: (I) Conception and design: JA Pawitan; (II) Administrative support: CE Nofianti, IN Sari; (III) Provision of study material or patients: Novialdi; (IV) Collection and assembly of data: CE Nofianti, IN Sari, Novialdi; (V) Data analysis and interpretation: Marlina, Novialdi, JA Pawitan; (VI) Manuscript writing: All authors; (VII) Final approval of manuscript: All authors.

          [#]

          These authors contributed equally to this work.

          Correspondence to: Jeanne Adiwinata Pawitan. Department of Histology, Faculty of Medicine Universitas Indonesia, Jl. Salemba 6, Jakarta 10430, Indonesia. Email: jeanneadiwip@ 123456gmail.com .
          Article
          PMC6044052 PMC6044052 6044052 sci-05-2018.05.04
          10.21037/sci.2018.05.04
          6044052
          30050919
          2018 Stem Cell Investigation. All rights reserved.
          Categories
          Original Article

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