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      A Morphometric Study of Age Changes in the Rat Optic Nerve

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          Abstract

          Age-related changes of the optic nerve fibres were studied in 3-month-old (young), 12-month-old (adult) and 24-month-old (aged) male Sprague-Dawley rats. The optic nerve was harvested with particular care from the intracranial portion. Cross sections from the optic nerve of animals of different age groups were stained with toluidine blue and examined under a light microscope at low and high magnification. Other sections were stained for the demonstration of glial cells using the method described by Holzer. A third group of sections were stained by the immunohistochemical method to detect glial fibrillary acidic protein, which is a marker for localising and characterising astrocytes. All these morphological results were subjected to the quantitative analysis of images and to statistical analysis of the values to identify significant morphometric data. Biochemical dosages of proteins were also performed on homogenised fragments of the optic nerve. Our results demonstrate that the following age-related changes can be observed: (1) an increase in meningeal membranes, (2) an increased number of astrocytes, (3) an increase in areal density of glial fibrillary acidic protein immunoreactivities, (4) an increased thickness of the entire optic nerve and an increased area of the nerve, (5) a decreased number of nerve fibres and (6) a decrease in the nerve fibre/meningeal membrane ratio from 3:1 to 1:1. Moreover, the amount of protein does not change with age. The rat optic nerve, therefore, appears sensitive to aging processes.

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          Most cited references4

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          Evidence of cell loss from the rat retina during senescence.

          In order to determine whether neuronal cell death in the retina accompanies senescence, retinas from pigmented ACI rats of various ages were analysed for changes in dimensions, cell densities, DNA content, and rhodopsin levels. Between the ages of 4 and 30 months, there were significant declines in the densities of nuclei in all three nuclear layers of the retina. Over this age span, nuclear density in the outer nuclear layer (ONL) declined by 32-41%, depending on the region of the retina examined, while inner nuclear layer (INL) nuclear density decreased by 25-34%. Over the same age range, the nuclear density in the ganglion cell layer (GCL) declined by 25-28%. The eye increased in size over the 4-30 month age span, particularly between 4 and 12 months. The retinal stretching that resulted from this growth could only partially account for the age-related decline in retinal cell densities. Depending on the region of the retina examined, decreases in nuclear density of 17-26% for the ONL, 9-17% for the INL, and 16-20% for the GCL were apparently due to cell death. Measurements of retinal DNA content support the conclusion that significant cell loss from the retina occurs during senescence; between 4 and 30 months of age retinal DNA content decreased by about 13%. Age-related changes in retinal rhodopsin content did not parallel apparent changes in photoreceptor number. Between 4 and 11 months of age, rhodopsin levels rose by about 10%, and then remained constant up to 26 months. Over this same age span, there was an apparent decrease in photoreceptor number of about 15%.(ABSTRACT TRUNCATED AT 250 WORDS)
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            • Record: found
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            Glial fibrillary acidic protein in cryogenic lesions of the rat brain

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              • Record: found
              • Abstract: not found
              • Article: not found

              Electrical activity of lateral geniculate nucleus and optic tract of the rat

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                Author and article information

                Journal
                OPH
                Ophthalmologica
                10.1159/issn.0030-3755
                Ophthalmologica
                S. Karger AG
                0030-3755
                1423-0267
                2001
                October 2001
                17 August 2001
                : 215
                : 5
                : 366-371
                Affiliations
                aDepartment of Neurosciences, bDepartment of Cardiovascular and Respiratory Sciences and cEye Clinic, University of Rome ‘La Sapienza’, Italy
                Article
                50887 Ophthalmologica 2001;215:366–371
                10.1159/000050887
                11528263
                5c0d4820-e434-44be-81a6-dd59b6da781c
                © 2001 S. Karger AG, Basel

                Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

                History
                Page count
                Figures: 3, Tables: 2, References: 28, Pages: 6
                Categories
                Original Paper · Travail original · Originalarbeit

                Vision sciences,Ophthalmology & Optometry,Pathology
                Histology,Aging,Optic nerve
                Vision sciences, Ophthalmology & Optometry, Pathology
                Histology, Aging, Optic nerve

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