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      Submersible Spectrofluorometer for Real-Time Sensing of Water Quality

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          Abstract

          In this work, we present a newly developed submersible spectrofluorometer (patent pending) applied to real-time sensing of water quality, suitable for monitoring some important indicators of the ecological status of natural waters such as chlorophyll-a, oil and protein-like material. For the optomechanical realization of the apparatus, a novel conceptual design has been adopted in order to avoid filters and pumps while maintaining a high signal-to-noise ratio. The elimination of filters and pumps has the advantage of greater system simplicity and especially of avoiding the risk of sample degradation. The use of light-emitting diodes as an excitation source instead of Xe lamps or laser diodes helped save on size, weight, power consumption and costs. For sensor calibration we performed measurements on water samples with added chlorophyll prepared in the laboratory. The sensor functionality was tested during field campaigns conducted at Albano Lake in Latium Region of Italy as well as in the Herzliya Harbor, a few kilometers North East of Tel Aviv in Israel. The obtained results are reported in the paper. The sensitivity achieved for chlorophyll-a detection was found to be at least 0.2 µg/L.

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          Fluorescence spectroscopy opens new windows into dissolved organic matter dynamics in freshwater ecosystems: A review

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            Seeing the light: The effects of particles, dissolved materials, and temperature on in situ measurements of DOM fluorescence in rivers and streams

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              In situ Measurements of Phytoplankton Fluorescence Using Low Cost Electronics

              Chlorophyll a fluorometry has long been used as a method to study phytoplankton in the ocean. In situ fluorometry is used frequently in oceanography to provide depth-resolved estimates of phytoplankton biomass. However, the high price of commercially manufactured in situ fluorometers has made them unavailable to some individuals and institutions. Presented here is an investigation into building an in situ fluorometer using low cost electronics. The goal was to construct an easily reproducible in situ fluorometer from simple and widely available electronic components. The simplicity and modest cost of the sensor makes it valuable to students and professionals alike. Open source sharing of architecture and software will allow students to reconstruct and customize the sensor on a small budget. Research applications that require numerous in situ fluorometers or expendable fluorometers can also benefit from this study. The sensor costs US$150.00 and can be constructed with little to no previous experience. The sensor uses a blue LED to excite chlorophyll a and measures fluorescence using a silicon photodiode. The sensor is controlled by an Arduino microcontroller that also serves as a data logger.
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                Author and article information

                Contributors
                Role: Academic Editor
                Journal
                Sensors (Basel)
                Sensors (Basel)
                sensors
                Sensors (Basel, Switzerland)
                MDPI
                1424-8220
                18 June 2015
                June 2015
                : 15
                : 6
                : 14415-14434
                Affiliations
                Diagnostics and Metrology Laboratory, ENEA, Via Enrico Fermi 45, 00044 Frascati, Italy; E-Mails: luca.fiorani@ 123456enea.it (L.F.); ivano.menicucci@ 123456enea.it (I.M.); marco.pistilli@ 123456enea.it (M.P.); antonia.lai@ 123456enea.it (A.L.)
                Author notes
                [* ]Author to whom correspondence should be addressed; E-Mail: adriana.puiu@ 123456enea.it ; Tel.: +39-6-9400-5498; Fax: +39-6-9400-5312.
                Article
                sensors-15-14415
                10.3390/s150614415
                4507635
                26094628
                5c450eb3-da02-4461-93e1-5ee36ddaf9fc
                © 2015 by the authors; licensee MDPI, Basel, Switzerland.

                This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license ( http://creativecommons.org/licenses/by/4.0/).

                History
                : 23 March 2015
                : 16 June 2015
                Categories
                Article

                Biomedical engineering
                light-induced fluorescence,spectrofluorometric sensor,water quality,chlorophyll detection

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