Soil and soil microbial biomass (SMB) carbon: nitrogen: phosphorus (C:N:P) stoichiometry are important parameters to determine soil balance of nutrients and circulation of materials, but how soil and SMB C:N:P stoichiometry is affected by climate change remains unclear. Field experiments with warming and N addition had been implemented since April 2007. Infrared radiators were used to manipulate temperature, and aqueous ammonium nitrate (10 g m −2 yr −1) was added to simulate nitrogen deposition. We found that molar nutrient ratios in the soil averaged 60:11:1, warming and warming plus N addition reduced soil C:N by 14.1% and 20% ( P < 0.01), and reduced soil C:P ratios by 14.5% and 14.8% ( P < 0.01). N addition reduced soil C:N significantly by 17.6% ( P < 0.001) (Figure 2B,D). N addition and warming plus N addition increased soil N:P significantly by 24.6% and 7.7% ( P < 0.01). The SMB C:N, C:P and N:P ratios increased significantly with warming, N addition and warming plus N addition. Warming and N addition increased the correlations between SOC and soil microbial biomass C (SMBC), soil total P and soil microbial biomass P (SMBP), warming increased the correlation between the soil total N and soil microbial biomass N (SMBN). After four years’ treatment, our results demonstrated that the combined effects of warming and N fertilization could change the C, N, P cycling by affecting soil and SMB C:N:P ratios significantly and differently. At the same time, our results suggested SMB might have weak homeostasis in Sonnen Grassland and warming and N addition would ease N-limitation but aggravate P-limitation in northeastern China. Furthermore, these results further the current demonstration of the relationships between the soil and SMB C:N:P stoichiometry in response to global change in temperate grassland ecosystems.